Complete genome sequence of grapevine yellow speckle viroid 3, a novel apscaviroid infecting grapevine, characterized by high-throughput sequencing.
Arch Virol
; 169(10): 194, 2024 Sep 09.
Article
in En
| MEDLINE
| ID: mdl-39249561
ABSTRACT
A novel grapevine viroid was discovered in an asymptomatic grapevine of Indian rootstocks. The whole genome sequence of the viroid (370 nt) was determined by high-throughput sequencing as well as RT-PCR followed by cloning and Sanger sequencing. The terminal conserved region (TCR), central conserved region (CCR) upper strand, and CCR lower strand are conserved regions found in the viroid that are unique to the members of the genus Apscaviroid. Based on our findings and the demarcation criteria for viroids, the novel viroid, which we have tentatively named "grapevine yellow speckle viroid 3" is a putative new member of the genus Apscaviroid.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phylogeny
/
Plant Diseases
/
Viroids
/
Genome, Viral
/
Vitis
/
High-Throughput Nucleotide Sequencing
Language:
En
Journal:
Arch Virol
/
Arch. virol
/
Archives of virology
Year:
2024
Document type:
Article
Affiliation country:
India
Country of publication:
Austria