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Strategies for producing probiotic biomass and postbiotics from Akkermansia muciniphila in submerged cultivations incorporating prebiotic sources.
Montipó, Sheila; Menegussi, Elisa Bellan; Fontana, Roselei Claudete; Camassola, Marli.
Affiliation
  • Montipó S; Biotechnology Institute, University of Caxias do Sul, Caxias do Sul, 95070-560, Brazil. smontipo@ucs.br.
  • Menegussi EB; Biotechnology Institute, University of Caxias do Sul, Caxias do Sul, 95070-560, Brazil.
  • Fontana RC; Biotechnology Institute, University of Caxias do Sul, Caxias do Sul, 95070-560, Brazil.
  • Camassola M; Biotechnology Institute, University of Caxias do Sul, Caxias do Sul, 95070-560, Brazil.
World J Microbiol Biotechnol ; 40(10): 314, 2024 Sep 09.
Article in En | MEDLINE | ID: mdl-39249571
ABSTRACT
This research propounds an innovative technology focused on sustainability to increase the biomass yield of Akkermansia muciniphila, the next-generation probiotic, using prebiotic sources to replace or reduce animal mucin levels. A series of experimental design approaches were developed aiming to optimize the growth of Akkermansiamuciniphila by incorporating extracts of green leafy vegetables and edible mushroom into the cultivation media. Experiments using kale extract (KE), Brassica oleracea L., associated with lyophilized mushroom extract (LME) of Pleurotus ostreatus were the most promising, highlighting the assays with 0.376% KE and 0.423% LME or 1.05% KE and 0.5% LME, in which 3.5 × 1010 CFU (Colony Forming Units) mL- 1 was achieved - higher than in experiments in optimized synthetic media. Such results enhance the potential of using KE and LME not only as mucin substitutes, but also as a source to increase Akkermansia muciniphila biomass yields and release short-chain fatty acids. The work is relevant to the food and pharmaceutical industries in the preparation of the probiotic ingredient.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Biomass / Probiotics / Culture Media / Prebiotics / Verrucomicrobia / Akkermansia Language: En Journal: World J Microbiol Biotechnol Year: 2024 Document type: Article Affiliation country: Brazil Country of publication: Germany

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Biomass / Probiotics / Culture Media / Prebiotics / Verrucomicrobia / Akkermansia Language: En Journal: World J Microbiol Biotechnol Year: 2024 Document type: Article Affiliation country: Brazil Country of publication: Germany