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α2,6 sialylation distinguishes a novel active state in CD4+ and CD8+ cells during acute Toxoplasma gondii infection.
Sierra-Ulloa, Diego; Fernández, Jacquelina; Cacelín, María; González-Aguilar, Gloria A; Saavedra, Rafael; Tenorio, Eda P.
Affiliation
  • Sierra-Ulloa D; Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, Mexico.
  • Fernández J; Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Mexico City, Mexico.
  • Cacelín M; Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, Mexico.
  • González-Aguilar GA; Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, Mexico.
  • Saavedra R; Posgrado en Ciencias Biológicas, Universidad Nacional Autónoma de México, Mexico City, Mexico.
  • Tenorio EP; Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Mexico City, Mexico.
Front Immunol ; 15: 1429302, 2024.
Article in En | MEDLINE | ID: mdl-39253089
ABSTRACT
Toxoplasmosis is a worldwide parasitosis that is usually asymptomatic; cell-mediated immunity, particularly T cells, is a crucial mediator of the immune response against this parasite. Membrane protein expression has been studied for a long time in T lymphocytes, providing vital information to determine functional checkpoints. However, less is known about the role of post-translational modifications in T cell function. Glycosylation plays essential roles during maturation and function; particularly, sialic acid modulation is determinant for accurate T cell regulation of processes like adhesion, cell-cell communication, and apoptosis induction. Despite its importance, the role of T cell sialylation during infection remains unclear. Herein, we aimed to evaluate whether different membrane sialylation motifs are modified in T cells during acute Toxoplasma gondii infection using different lectins. To this end, BALB/c Foxp3EGFP mice were infected with T. gondii, and on days 3, 7, and 10 post-infection, splenocytes were obtained to analyze conventional (Foxp3-) CD4+ and CD8+ populations by flow cytometry. Among the different lectins used for analysis, only Sambucus nigra lectin, which detects sialic acid α2,6 linkages, revealed two distinctive populations (SNBright and SN-/Dim) after infection. Further characterization of CD4+ and CD8+ SN-/Dim lymphocytes showed that these are highly activated cells, with a TEf/EM or TCM phenotype that produce high IFN-γ levels, a previously undescribed cell state. This work demonstrates that glycan membrane analysis in T cells reveals previously overlooked functional states by evaluating only protein expression.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Toxoplasma / CD4-Positive T-Lymphocytes / Toxoplasmosis / CD8-Positive T-Lymphocytes / Mice, Inbred BALB C Limits: Animals Language: En Journal: Front Immunol Year: 2024 Document type: Article Affiliation country: Mexico Country of publication: Switzerland

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Toxoplasma / CD4-Positive T-Lymphocytes / Toxoplasmosis / CD8-Positive T-Lymphocytes / Mice, Inbred BALB C Limits: Animals Language: En Journal: Front Immunol Year: 2024 Document type: Article Affiliation country: Mexico Country of publication: Switzerland