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The Relationship between HERV, Interleukin, and Transcription Factor Expression in ZIKV Infected versus Uninfected Trophoblastic Cells.
Costa, Anderson Luís da; Prieto-Oliveira, Paula; Duarte-Barbosa, Márcia; Andreata-Santos, Robert; Peter, Cristina M; Prolo de Brito, Thamires; Antoneli, Fernando; Durães-Carvalho, Ricardo; Briones, Marcelo R S; Maricato, Juliana T; Zanotto, Paolo M A; Jacob Machado, Denis; Janini, Luiz M R.
Affiliation
  • Costa ALD; Laboratory of Retrovirology, Discipline of Infectology, Department of Medicine, Federal University of São Paulo (EPM-UNIFESP), São Paulo 04039-032, Brazil.
  • Prieto-Oliveira P; Department of Bioinformatics and Genomics, College of Computing and Informatics, University of North Carolina at Charlotte, 9331 Robert D. Snyder Rd., Charlotte, NC 28223, USA.
  • Duarte-Barbosa M; Computational Intelligence to Predict Health and Environmental Risks Center, University of North Carolina at Charlotte, 9201 University City BLVD, Charlotte, NC 28223, USA.
  • Andreata-Santos R; Laboratory of Retrovirology, Discipline of Infectology, Department of Medicine, Federal University of São Paulo (EPM-UNIFESP), São Paulo 04039-032, Brazil.
  • Peter CM; Laboratory of Retrovirology, Discipline of Microbiology, Department of Microbiology, Immunology and Parasitology, Federal University of São Paulo, São Paulo 04039-032, Brazil.
  • Prolo de Brito T; Laboratory of Retrovirology, Discipline of Microbiology, Department of Microbiology, Immunology and Parasitology, Federal University of São Paulo, São Paulo 04039-032, Brazil.
  • Antoneli F; Center for Medical Bioinformatics, Federal University of São Paulo, São Paulo 04039-032, Brazil.
  • Durães-Carvalho R; Laboratory of Retrovirology, Discipline of Microbiology, Department of Microbiology, Immunology and Parasitology, Federal University of São Paulo, São Paulo 04039-032, Brazil.
  • Briones MRS; Center for Medical Bioinformatics, Federal University of São Paulo, São Paulo 04039-032, Brazil.
  • Maricato JT; Laboratory of Retrovirology, Discipline of Microbiology, Department of Microbiology, Immunology and Parasitology, Federal University of São Paulo, São Paulo 04039-032, Brazil.
  • Zanotto PMA; Department of Morphology and Genetics, Federal University of São Paulo, São Paulo 04039-032, Brazil.
  • Jacob Machado D; Center for Medical Bioinformatics, Federal University of São Paulo, São Paulo 04039-032, Brazil.
  • Janini LMR; Laboratory of Retrovirology, Discipline of Microbiology, Department of Microbiology, Immunology and Parasitology, Federal University of São Paulo, São Paulo 04039-032, Brazil.
Cells ; 13(17)2024 Sep 05.
Article in En | MEDLINE | ID: mdl-39273061
ABSTRACT
Zika virus (ZIKV) is an arbovirus with maternal, sexual, and TORCH-related transmission capabilities. After 2015, Brazil had the highest number of ZIVK-infected pregnant women who lost their babies or delivered them with Congenital ZIKV Syndrome (CZS). ZIKV triggers an immune defense in the placenta. This immune response counts with the participation of interleukins and transcription factors. Additionally, it has the potential involvement of human endogenous retroviruses (HERVS). Interleukins are immune response regulators that aid immune tolerance and support syncytial structure development in the placenta, where syncytin receptors facilitate vital cell-to-cell fusion events. HERVs are remnants of ancient viral infections that integrate into the genome and produce syncytin proteins crucial for placental development. Since ZIKV can infect trophoblast cells, we analyzed the relationship between ZIKV infection, HERV, interleukin, and transcription factor modulations in the placenta. To investigate the impact of ZIKV on trophoblast cells, we examined two cell types (BeWo and HTR8) infected with ZIKV-MR766 (African) and ZIKV-IEC-Paraíba (Asian-Brazilian) using Taqman and RT2 Profiler PCR Array assays. Our results indicate that early ZIKV infection (24-72 h) does not induce differential interleukins, transcription factors, and HERV expression. However, we show that the expression of a few of these host defense genes appears to be linked independently of ZIKV infection. Future studies involving additional trophoblastic cell lineages and extended infection timelines will illuminate the dynamic interplay between ZIKV, HERVs, interleukins, and transcription factors in the placenta.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Transcription Factors / Trophoblasts / Interleukins / Endogenous Retroviruses / Zika Virus / Zika Virus Infection Limits: Female / Humans / Pregnancy Language: En Journal: Cells Year: 2024 Document type: Article Affiliation country: Brazil Country of publication: Switzerland

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Transcription Factors / Trophoblasts / Interleukins / Endogenous Retroviruses / Zika Virus / Zika Virus Infection Limits: Female / Humans / Pregnancy Language: En Journal: Cells Year: 2024 Document type: Article Affiliation country: Brazil Country of publication: Switzerland