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Mesenchymal Stem/Stromal Cells Derived from Dental Tissues Mediate the Immunoregulation of T Cells through the Purinergic Pathway.
Poblano-Pérez, Luis Ignacio; Monroy-García, Alberto; Fragoso-González, Gladis; Mora-García, María de Lourdes; Castell-Rodríguez, Andrés; Mayani, Héctor; Álvarez-Pérez, Marco Antonio; Pérez-Tapia, Sonia Mayra; Macías-Palacios, Zaira; Vallejo-Castillo, Luis; Montesinos, Juan José.
Affiliation
  • Poblano-Pérez LI; Mesenchymal Stem Cell Laboratory, Oncology Research Unit, Oncology Hospital, Centro Médico Nacional SXXI, Instituto Mexicano del Seguro Social, Mexico City 06720, Mexico.
  • Monroy-García A; Programa de Doctorado en Ciencias Biomédicas, Universidad Nacional Autónoma de México, Mexico City 04510, Mexico.
  • Fragoso-González G; Immunology and Cancer Laboratory, Oncology Research Unit, Oncology Hospital, Centro Médico Nacional SXXI, Instituto Mexicano del Seguro Social, Mexico City 06720, Mexico.
  • Mora-García ML; Institute of Biomedical Research, Department of Immunology, Universidad Nacional Autónoma de México, Mexico City 04510, Mexico.
  • Castell-Rodríguez A; Immunobiology Laboratory, Cell Differentiation and Cancer Unit, Facultad de Estudios Superiores-Zaragoza, Universidad Nacional Autónoma de México, Mexico City 09230, Mexico.
  • Mayani H; Department of Cellular and Tissue Biology, Faculty of Medicine, Universidad Nacional Autónoma de México, Mexico City 04510, Mexico.
  • Álvarez-Pérez MA; Hematopoietic Stem Cell Laboratory, Oncology Research Unit, Oncology Hospital, Centro Médico Nacional SXXI, Instituto Mexicano del Seguro Social, Mexico City 06720, Mexico.
  • Pérez-Tapia SM; Tissue Bioengineering Laboratory, Postgraduate Studies, Research Division, Faculty of Dentistry, Universidad Nacional Autónoma de México, Mexico City 04510, Mexico.
  • Macías-Palacios Z; Research and Development in Biotherapeutic Unit (UDIBI), National School of Biological Sciences, Instituto Politécnico Nacional, Mexico City 11340, Mexico.
  • Vallejo-Castillo L; National Laboratory for Specialized Services of Investigation, Development and Innovation (I+D+i) for Pharma Chemicals and Biotechnological Products (LANSEIDI-FarBiotec-CONACyT), Instituto Politécnico Nacional, Mexico City 11340, Mexico.
  • Montesinos JJ; Department of Immunology, National School of Biological Sciences, Instituto Politécnico Nacional, Mexico City 11340, Mexico.
Int J Mol Sci ; 25(17)2024 Sep 04.
Article in En | MEDLINE | ID: mdl-39273524
ABSTRACT
Human dental tissue mesenchymal stem cells (DT-MSCs) constitute an attractive alternative to bone marrow-derived mesenchymal stem cells (BM-MSCs) for potential clinical applications because of their accessibility and anti-inflammatory capacity. We previously demonstrated that DT-MSCs from dental pulp (DP-MSCs), periodontal ligaments (PDL-MSCs), and gingival tissue (G-MSCs) show immunosuppressive effects similar to those of BM, but to date, the DT-MSC-mediated immunoregulation of T lymphocytes through the purinergic pathway remains unknown. In the present study, we compared DP-MSCs, PDL-MSCs, and G-MSCs in terms of CD26, CD39, and CD73 expression; their ability to generate adenosine (ADO) from ATP and AMP; and whether the concentrations of ADO that they generate induce an immunomodulatory effect on T lymphocytes. BM-MSCs were included as the gold standard. Our results show that DT-MSCs present similar characteristics among the different sources analyzed in terms of the properties evaluated; however, interestingly, they express more CD39 than BM-MSCs; therefore, they generate more ADO from ATP. In contrast to those produced by BM-MSCs, the concentrations of ADO produced by DT-MSCs from ATP inhibited the proliferation of CD3+ T cells and promoted the generation of CD4+CD25+FoxP3+CD39+CD73+ Tregs and Th17+CD39+ lymphocytes. Our data suggest that DT-MSCs utilize the adenosinergic pathway as an immunomodulatory mechanism and that this mechanism is more efficient than that of BM-MSCs.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Periodontal Ligament / Apyrase / T-Lymphocytes / 5'-Nucleotidase / Adenosine / Dental Pulp / Mesenchymal Stem Cells Limits: Humans Language: En Journal: Int J Mol Sci Year: 2024 Document type: Article Affiliation country: Mexico Country of publication: Switzerland

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Periodontal Ligament / Apyrase / T-Lymphocytes / 5'-Nucleotidase / Adenosine / Dental Pulp / Mesenchymal Stem Cells Limits: Humans Language: En Journal: Int J Mol Sci Year: 2024 Document type: Article Affiliation country: Mexico Country of publication: Switzerland