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AXL inhibition prevents RPA2/CHK1-mediated homologous recombination to increase PARP inhibitor sensitivity in hepatocellular carcinoma.
Li, Kai-Min; Deng, Li-Gong; Xue, Li-Jun; Tan, Chang; Yao, Shu-Kun.
Affiliation
  • Li KM; School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191, China.
  • Deng LG; School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191, China.
  • Xue LJ; Department of Gastroenterology, Jinan Central Hospital Affiliated to Shandong First Medical University, Jinan, 250013, Shandong Province, China.
  • Tan C; Graduate School, Peking University China-Japan Friendship School of Clinical Medicine, Beijing, 100029, China.
  • Yao SK; School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191, China.
Heliyon ; 10(17): e36283, 2024 Sep 15.
Article in En | MEDLINE | ID: mdl-39281567
ABSTRACT
Homologous recombination defects (HRD) render cells fail to repair DNA double-strand break (DSB), which causes synthetic lethality in these cells with punch by poly (ADP-ribose) polymerase (PARP) inhibitors (PARPi). Here, we reveal a receptor tyrosine kinase, AXL, whose inhibition leads to HRD in hepatocellular carcinoma (HCC) cells. AXL is upregulated in HCC tumors, which is positively correlated with low survival rates. AXL knockdown or AXL inhibition by bemcentinib reduces HR efficiency in HCC cells, and AXL plays its role in HR repair through its kinase activity. Furthermore, we find that AXL interacts with RPA2, enhancing the recruitment of RPA2 to DNA damage sites. Mechanistically, AXL promotes the tyrosinization of RPA2 at tyrosine 9, promoting the phosphorylation of CHK1, thereby strengthens the HR repair ability in HCC cells to resist DNA damage. In conclusion, our results reveal that AXL is a promising therapeutic biomarker for HCC patients, and present that targeting AXL-RPA2-CHK1 pathway together with PARP inhibitor will be effective therapeutic strategy in HCC.

Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Heliyon Year: 2024 Document type: Article Affiliation country: China Country of publication: United kingdom

Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Heliyon Year: 2024 Document type: Article Affiliation country: China Country of publication: United kingdom