Single GC base pair recognition by a heterocyclic diamidine: structures, affinities, and dynamics.
RSC Adv
; 14(40): 29675-29682, 2024 Sep 12.
Article
in En
| MEDLINE
| ID: mdl-39297050
ABSTRACT
The recognition of specific genomic arrangements by rationally designed small molecules is fundamental for the expansion of targeted gene expression. Here, we report the first X-ray crystal structures that demonstrate single G (guanine) recognition by a highly selective diamidine (DB2447) in a mixed DNA sequence. The study presents detailed structural information on the mechanism of single G recognition by D2447 and its various interactions in the DNA minor groove. Molecular dynamics and binding studies were used to evaluate the details of our reported structures. The study provides structural insight and resources necessary for understanding single G selection in genomic sequences.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
RSC Adv
Year:
2024
Document type:
Article
Country of publication:
United kingdom