Effect and mechanism of Lycium barbarum polysaccharide on gastrointestinal motility in slow transit constipation.

ABSTRACT

Slow transit constipation (STC) seriously affects the physical and mental health of patients. While the active ingredients of traditional Chinese medicine (TCM) are widely used in the treatment of STC due to their low toxicity and side effects, the aim of this study was to investigate the effect of Lycium barbarum polysaccharide (LBP) on STC. The STC mouse model was induced by the compound diphenoxylate. Defecation, fecal moisture, and weight loss of the STC models were monitored. Gastrointestinal (GI) motility was assessed by intestinal propulsive rate, and enzyme-linked immunosorbent assay (ELISA) kits were used to analyze the levels of substance P (SP) and vasoactive intestinal peptide (VIP). The expression levels of inflammatory cytokines (Tnf-α, Il-6, and Il-1ß), stem cell factor receptor (C-kit), stem cell factor (Scf), Bcl-2, Bax, and Caspase-3 were evaluated by qRT-PCR. The defecation, fecal moisture, and body weight of mice with STC were significantly improved by LBP, and LBP increased the intestinal propulsive rate of STC, increased the secretion of SP, and decreased the secretion of VIP. The intervention of LBP further suppressed the expression levels of Tnf-α, Il-6, and Il-1ß in STC. LBP promoted the expression of the C-kit, Scf, and Bcl-2 and inhibited the expression of Bax and Caspase-3. LBP may alleviate symptoms of slow transit constipation (STC) and enhance gastrointestinal motility by modulating gastrointestinal hormone levels, promoting proliferation, and inhibiting the apoptosis of interstitial cells of Cajal (ICCs).