Long-term grazing reduces soil fungal network complexity but enhances plant-soil microbe network connectivity in a semi-arid grassland.

ABSTRACT

Grazing plays a significant role in shaping both aboveground vegetation and belowground microbial communities in arid and semi-arid grasslands, which in turn affects ecosystem functions and sustainability. Therefore, it was essential to implement effective grazing management practices to preserve ecological balance and support sustainable development in these delicate environments. To optimize the traditional continuous grazing policy, we conducted a 10-year seasonal grazing experiment with five treatments in a typical grassland in northern China no grazing (NG), continuous summer grazing (CG), and three seasonal grazing treatments (G57 in May and July, G68 in June and August, and G79 in July and September). Our study found that although grazing reduced plant community biomass, G68 treatment maintained high plant height and community diversity (P < 0.05). Grazing did not affect soil bacterial and archaeal alpha diversity, but CG treatment reduced soil fungal diversity (P < 0.05). CG reduced the archaeal network's vertices (which represent microbial taxa, OTUs) and connections (ecological interactions between taxa), but seasonal grazing increased its complexity. Furthermore, grazing did not change bacterial networks but enhanced cross-domain interactions (relationships between different biological groups) of plant-soil fungi and plant-soil archaea. Overall, we used the Mantel test to find that soil microbial diversity was positively correlated with soil physicochemical properties rather than plant community characteristics after grazing. These findings are beneficial for the optimization of sustainable grassland management policies and the protection of plant and soil biodiversity.