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Candidate antibody reference reagents for Chlamydia trachomatis serology.
da Silva, Filomeno Coelho; Kamuyu, Gathoni; Michels, Birgitta; Edney, Jessica; Hassall, Laura; Stickings, Paul; Maharjan, Sunil; Waterboer, Tim; Beddows, Simon.
Affiliation
  • da Silva FC; Virus Reference Department, Public Health Microbiology Division, UK Health Security Agency, London, UK.
  • Kamuyu G; Virus Reference Department, Public Health Microbiology Division, UK Health Security Agency, London, UK.
  • Michels B; Infections and Cancer Epidemiology, German Cancer Research Center (DKFZ), Heidelberg, Germany.
  • Edney J; Virus Reference Department, Public Health Microbiology Division, UK Health Security Agency, London, UK.
  • Hassall L; Medicines and Healthcare products Regulatory Agency (MHRA), National Institute for Biological Standards and Control (NIBSC), South Mimms, Potters Bar, UK.
  • Stickings P; Medicines and Healthcare products Regulatory Agency (MHRA), National Institute for Biological Standards and Control (NIBSC), South Mimms, Potters Bar, UK.
  • Maharjan S; Medicines and Healthcare products Regulatory Agency (MHRA), National Institute for Biological Standards and Control (NIBSC), South Mimms, Potters Bar, UK.
  • Waterboer T; Infections and Cancer Epidemiology, German Cancer Research Center (DKFZ), Heidelberg, Germany.
  • Beddows S; Virus Reference Department, Public Health Microbiology Division, UK Health Security Agency, London, UK; Blood Safety, Hepatitis, Sexually Transmitted Infections and HIV Division, UK Health Security Agency, London, UK. Electronic address: simon.beddows@ukhsa.gov.uk.
J Immunol Methods ; 534: 113761, 2024 Sep 24.
Article in En | MEDLINE | ID: mdl-39326782
ABSTRACT
Chlamydia trachomatis (Ct) serology is an important tool for monitoring infection and disease burden but there are currently no formal reference reagents to harmonize results reporting. Our objective was to develop a panel of candidate reference reagents with reactivity against the major outer membrane protein (MOMP) and virulence factor (pgp3) antigens. Plasma packs from females (20-40 years old) were screened against MOMP and pgp3 antigens and selected positive and negative samples pooled to create a panel of candidate antibody reference reagents that were tested in two laboratories. Antigen specificity and internal quality assurance were also evaluated. Suitable candidate materials have been selected to produce Ct reference reagents.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: J Immunol Methods Year: 2024 Document type: Article Affiliation country: United kingdom Country of publication: Netherlands
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Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: J Immunol Methods Year: 2024 Document type: Article Affiliation country: United kingdom Country of publication: Netherlands