High-speed reflectance confocal microscopy using speckle modulation.
Biomed Opt Express
; 15(8): 4877-4890, 2024 Aug 01.
Article
in En
| MEDLINE
| ID: mdl-39347009
ABSTRACT
We developed a spectrally-encoded, line reflectance confocal microscope (RCM) that uses a rotating diffuser to rapidly modulate the illumination speckle pattern. The speckle modulation approach reduced speckle noise while imaging with a spatially coherent light source needed for high imaging speed and cellular resolution. The speckle-modulation RCM device achieved lateral and axial resolutions of 1.1 µm and 2.8 µm, respectively. With an imaging speed of 107 frames/sec, three-dimensional RCM imaging over 300-µm depth was completed within less than 1 second. RCM images of human fingers, forearms, and oral mucosa clearly visualized the characteristic cellular features without any noticeable speckle noise.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Biomed Opt Express
Year:
2024
Document type:
Article
Affiliation country:
United States
Country of publication:
United States