Detection of Tn-antigen in breast and prostate cancer models by VVL-labeled red dye-doped nanoparticles.
Nanomedicine (Lond)
; : 1-16, 2024 Oct 09.
Article
in En
| MEDLINE
| ID: mdl-39382009
ABSTRACT
Aim:
Fluorescence detection of breast and prostate cancer cells expressing Tn-antigen, a tumor marker, with Vicia villosa lectin (VVL)-labeled nanoparticles.Materials &methods:
Breast and prostate cancer cells engineered to express high levels of Tn-antigen and non-engineered controls were incubated with VVL-labeled or unlabeled red dye-doped silica-coated polystyrene nanoparticles. The binding to cells was studied with flow cytometry, confocal microscopy, and electron microscopy.Results:
Flow cytometry showed that the binding of VVL-labeled nanoparticles was significantly higher to Tn-antigen-expressing cancer cells than controls. Confocal microscopy demonstrated that particles bound to the cell surface. According to the correlative light and electron microscopy the particles bound mostly as aggregates.Conclusion:
VVL-labeled nanoparticles could provide a new tool for the detection of Tn-antigen-expressing breast and prostate cancer cells.
[Box see text].
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Nanomedicine (Lond)
/
Nanomedicine (London. Online)
Year:
2024
Document type:
Article
Affiliation country:
Finland
Country of publication:
United kingdom