Can Immature Granulocyte Counting be Utilized in the Selection of Samples for Flow Cytometric Enumeration of Stem Cells?
Clin Lab
; 70(10)2024 Oct 01.
Article
in En
| MEDLINE
| ID: mdl-39382934
ABSTRACT
BACKGROUND:
This study aimed to compare the IMG counting by an auto hematology analyzer with the flow cytometric enumeration of CD34+ cells.METHODS:
All data from 124 samples submitted to the hematology laboratory for CD34+ cell counting in 2019 and 2020 were retrospectively evaluated. Whole blood samples were taken into EDTA tubes and assayed within 2 hours. The numbers and percentages of WBC and IMG were determined by using Mindray BC6200 hematology analyzer, while the same were determined for CD45 and CD34+ cells by using Beckman Coulter Navios flow cytometer. The performance of the new method was determined by the receiver operating characteristic (ROC) analysis.RESULTS:
Out of the 124 samples, 94 were collected from healthy individuals and 30 were collected from patients. A significant correlation was found between IMG and CD34+ cell counts in all patients (r = 0.71, p = 0.000) at the cutoff values of > 20/µL and > 50/µL. The Bland-Altman analysis of all patients showed that there was an agreement between the two methods. When the cutoff value of 20/µL for CD34+ cells was used in the ROC analysis, the sensitivity and specificity were calculated as 100 (96.1 - 100) and 96.77 (83.3 - 99.9), respectively, for the IMG count of > 900/µL.CONCLUSIONS:
An IMG count of 900/µL can be used as the cutoff value in the analysis with the Mindray BC6200. The IMG counting cannot replace the flow cytometric CD34+ cell enumeration but can be used in the selection of the samples for stem cell enumeration by flow cytometry.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Antigens, CD34
/
Flow Cytometry
Limits:
Adult
/
Aged
/
Female
/
Humans
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Male
/
Middle aged
Language:
En
Journal:
Clin Lab
/
Clin. lab
/
Clinical laboratory
Journal subject:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Year:
2024
Document type:
Article
Country of publication:
Germany