Your browser doesn't support javascript.
loading
Anti-Cancer Potential of Linear ß-(1→6)-D-Glucan from Agaricus bisporus on Estrogen Receptor-Positive (ER+) Breast Cancer Cells.
Rutckeviski, Renata; Corso, Claudia Rita; Fonseca, Aline Simoneti; Rodrigues, Mariane Londero; Román-Ochoa, Yony; Cipriani, Thales Ricardo; Cavalli, Luciane Regina; Cadena, Silvia Maria Suter Correia; Smiderle, Fhernanda Ribeiro.
Affiliation
  • Rutckeviski R; Faculdades Pequeno Príncipe, Curitiba 80230-020, PR, Brazil.
  • Corso CR; Instituto de Pesquisa Pelé Pequeno Príncipe, Avenida Munhoz da Rocha, 490, Curitiba 80035-000, PR, Brazil.
  • Fonseca AS; Faculdades Pequeno Príncipe, Curitiba 80230-020, PR, Brazil.
  • Rodrigues ML; Instituto de Pesquisa Pelé Pequeno Príncipe, Avenida Munhoz da Rocha, 490, Curitiba 80035-000, PR, Brazil.
  • Román-Ochoa Y; Instituto de Pesquisa Pelé Pequeno Príncipe, Avenida Munhoz da Rocha, 490, Curitiba 80035-000, PR, Brazil.
  • Cipriani TR; Departamento de Bioquímica Biologia Molecular, Universidade Federal do Paraná, Curitiba 81531-980, PR, Brazil.
  • Cavalli LR; Departamento de Bioquímica Biologia Molecular, Universidade Federal do Paraná, Curitiba 81531-980, PR, Brazil.
  • Cadena SMSC; Departamento de Bioquímica Biologia Molecular, Universidade Federal do Paraná, Curitiba 81531-980, PR, Brazil.
  • Smiderle FR; Faculdades Pequeno Príncipe, Curitiba 80230-020, PR, Brazil.
Molecules ; 29(19)2024 Oct 09.
Article in En | MEDLINE | ID: mdl-39407709
ABSTRACT
Mushroom ß-D-glucans can be isolated from several species, including the widely consumed Agaricus bisporus. Besides immunomodulatory responses, some ß-D-glucans may exhibit direct antitumoral effects. It was previously observed that a ß-(1→6)-D-glucan (BDG16) has indirect cytotoxicity on triple-negative breast cancer cells. In this study, the cytotoxicity of this same glucan was observed on estrogen receptor-positive (ER+) breast cancer cells (MCF-7). Cell viability was determined by multiple methods to assess metabolic activity, lysosomal membrane integrity, and adhesion capacity. Assays to evaluate cell respiration, cell cycle, apoptosis, necroptosis, and oxidative stress were performed to determine the action of BDG16 on MCF-7 cells. A gradual and significant cell viability reduction was observed when the cells were treated with BDG16 (10-1000 µg/mL). This result could be associated with the inhibition of the basal state respiration after incubation with the ß-D-glucan. The cells showed a significant arrest in G1 phase population at 1000 µg/mL, with no induction of apoptosis. However, an increase in necrosis and necroptosis at the same concentration was observed. No difference in oxidative stress-related molecules was observed. Altogether, our findings demonstrate that BDG16 directly induces toxicity in MCF-7 cells, primarily by impairing mitochondrial respiration and promoting necroptosis. The specific mechanisms that mediate this action are being investigated.
Subject(s)
Key words

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Agaricus / Breast Neoplasms / Receptors, Estrogen / Cell Survival / Apoptosis / Oxidative Stress / Antineoplastic Agents Limits: Female / Humans Language: En Journal: Molecules Journal subject: BIOLOGIA Year: 2024 Document type: Article Affiliation country: Brazil Country of publication: Switzerland

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Agaricus / Breast Neoplasms / Receptors, Estrogen / Cell Survival / Apoptosis / Oxidative Stress / Antineoplastic Agents Limits: Female / Humans Language: En Journal: Molecules Journal subject: BIOLOGIA Year: 2024 Document type: Article Affiliation country: Brazil Country of publication: Switzerland