On the use of affinity chromatography in demonstrating the transfer of thioester-bound D-phenylalanine from the light enzyme of gramicidin S synthetase to an acceptor site for this amino acid on the heavy enzyme.

Pass, L; Zimmer, T L; Laland, S G

Pass, L; Zimmer, T L; Laland, S G.

Eur J Biochem ; 47(3): 607-11, 1974 Sep 16.

Article in En | MEDLINE | ID: mdl-4140092

Subject(s)

Amino Acid Isomerases/metabolism; Gramicidin/biosynthesis; Multienzyme Complexes/metabolism; Peptide Synthases/metabolism; Phenylalanine/metabolism; Adenosine Triphosphate/metabolism; Amino Acids/metabolism; Binding Sites; Biological Transport; Carbon Radioisotopes; Chromatography, Affinity; Ligands; Models, Chemical; Phosphorus Radioisotopes; Protein Binding; Sulfhydryl Compounds/metabolism

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Collection: 01-internacional Database: MEDLINE Main subject: Peptide Synthases / Phenylalanine / Amino Acid Isomerases / Gramicidin / Multienzyme Complexes Language: En Journal: Eur J Biochem Year: 1974 Document type: Article

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