[Soluble proteins of the vegetative cells and spores of Clostridium botulinum type B and their toxicity]. / Rastvorimye belki vegetativnykh kletok i spor Clostridium botulinum tipa B i ikh toksichnost'

ABSTRACT

By disc microelectrophoresis in polyacrylamide gel proteins of vegetative cells and spores of Clostridium botulinum type B were investigated at different growth phases and toxicity of protein zones was measured. Protein components of vegetative cells varied in their electrophoretic mobility, depending on the growth phase, and differed significantly from spore proteins. Spores contained 1% toxin of vegetative cells. Electrophoregrams of intrasporal proteins showed a toxic zone with an approximate molecular weight of 10(5)-10(6).