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Inhibition of human HL-60 cell responses to chemotactic factors by antisense messenger RNA depletion of G proteins.
Goetzl, E J; Shames, R S; Yang, J; Birke, F W; Liu, Y F; Albert, P R; An, S.
Affiliation
  • Goetzl EJ; Department of Medicine, University of California Medical Center, San Francisco 94143-0711.
J Biol Chem ; 269(2): 809-12, 1994 Jan 14.
Article in En | MEDLINE | ID: mdl-8288630
Chemotactic factors bound to receptors of the seven-transmembrane domain family signal leukocytes through associated guanine nucleotide-binding (G) proteins. Human leukocytes of the HL-60 line, which express G protein-coupled receptors for leukotriene B4 (LTB4) and N-formyl-methionyl-leucyl-phenylalanine (fMLP) after differentiation with vitamin D3 and transforming growth factor-beta, were transfected with expression plasmids containing antisense-oriented cDNAs encoding the alpha-chains of Go, Gi1, Gi2, and Gi3. Antisense mRNA for Go and Gi2 alpha-chains suppressed by over 80% the level of the respective G protein. Go-deficient HL-60 cells had depressed functional and intracellular calcium responses to LTB4 and fMLP, but no alterations in the responses of cyclic adenosine 3',5'-monophosphate (cAMP). In contrast, HL-60 cells deficient in Gi2 lost only responses of the intracellular concentration of cAMP. Antisense mRNA suppression of distinct G proteins thus may delineate some transductional requirements for cellular responses.
Subject(s)
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Collection: 01-internacional Database: MEDLINE Main subject: Chemotactic Factors / Chemotaxis, Leukocyte / GTP-Binding Proteins Limits: Humans Language: En Journal: J Biol Chem Year: 1994 Document type: Article Country of publication: United States
Search on Google
Collection: 01-internacional Database: MEDLINE Main subject: Chemotactic Factors / Chemotaxis, Leukocyte / GTP-Binding Proteins Limits: Humans Language: En Journal: J Biol Chem Year: 1994 Document type: Article Country of publication: United States