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Expression of the human TSH receptor in a human thyroid carcinoma cell line that lacks an endogenous TSH receptor: growth inhibition by cAMP.
Derwahl, M; Kuemmel, M; Goretzki, P; Schatz, H; Broecker, M.
Affiliation
  • Derwahl M; Laboratory of Experimental Endocrinology, Clinic of Internal Medicine, University of Bochum, Germany.
Biochem Biophys Res Commun ; 191(3): 1131-8, 1993 Mar 31.
Article in En | MEDLINE | ID: mdl-8385451
The role of TSH and its receptor in controlling growth of thyroid carcinomas is far from well understood. In order to study this subject further we established a new human thyroid carcinoma cell line. We transfected human thyroid carcinoma cells lacking an endogenous TSH receptor with the human TSH receptor cDNA. Transfected cells, designated HTC-TSHr, expressed the TSH receptor mRNA and synthesized a functional TSH receptor with a TSH binding affinity in the order of magnitude of normal thyroid cells. In response to TSH stimulation HTC-TSHr cells accumulated cAMP, indicating a functional TSH receptor-adenylate cyclase system. However, HTC-TSHr cells did not concentrate iodide and lacked thyroglobulin immunoreactivity, although they did express low amounts of thyroglobulin mRNA. Proliferation of HTC-TSHr cells was inhibited by dibutyryl-cAMP and forskolin and also by TSH via the re-expressed TSH receptor.
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Collection: 01-internacional Database: MEDLINE Main subject: Receptors, Thyrotropin / Thyroid Neoplasms / Thyrotropin / Cyclic AMP Limits: Humans Language: En Journal: Biochem Biophys Res Commun Year: 1993 Document type: Article Affiliation country: Germany Country of publication: United States
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Collection: 01-internacional Database: MEDLINE Main subject: Receptors, Thyrotropin / Thyroid Neoplasms / Thyrotropin / Cyclic AMP Limits: Humans Language: En Journal: Biochem Biophys Res Commun Year: 1993 Document type: Article Affiliation country: Germany Country of publication: United States