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Precise deletions in large bacterial genomes by vector-mediated excision (VEX). The trfA gene of promiscuous plasmid RK2 is essential for replication in several gram-negative hosts.
Ayres, E K; Thomson, V J; Merino, G; Balderes, D; Figurski, D H.
Affiliation
  • Ayres EK; Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York, NY 10032.
J Mol Biol ; 230(1): 174-85, 1993 Mar 05.
Article in En | MEDLINE | ID: mdl-8450534
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Collection: 01-internacional Database: MEDLINE Main subject: Plasmids / Replicon / DNA Mutational Analysis / Genome, Bacterial / Sequence Deletion / Genes, Bacterial / Genetic Vectors / Gram-Negative Bacteria Language: En Journal: J Mol Biol Year: 1993 Document type: Article Country of publication: Netherlands
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Collection: 01-internacional Database: MEDLINE Main subject: Plasmids / Replicon / DNA Mutational Analysis / Genome, Bacterial / Sequence Deletion / Genes, Bacterial / Genetic Vectors / Gram-Negative Bacteria Language: En Journal: J Mol Biol Year: 1993 Document type: Article Country of publication: Netherlands