Monoclonal anti-biotin antibodies simulate avidin in the recognition of biotin.

Bagçi, H; Kohen, F; Kusçuoglu, U; Bayer, E A; Wilchek, M

Bagçi, H; Kohen, F; Kusçuoglu, U; Bayer, E A; Wilchek, M.

Affiliation

Bagçi H; Department of Hormone Research, Weizmann Institute of Science, Rehovot, Israel.

FEBS Lett ; 322(1): 47-50, 1993 May 03.

Article in En | MEDLINE | ID: mdl-8482366

ABSTRACT

The sequence of the VH gene of a monoclonal anti-biotin antibody was determined. Biotin-binding motifs, similar to those in avidin and streptavidin, were identified in complementary determining regions 2 and 3, suggesting that natural selection of functional motifs may occur in unrelated protein types.

Subject(s)

Antibodies, Monoclonal/immunology; Avidin/metabolism; Biotin/immunology; Immunoglobulin Heavy Chains/genetics; Immunoglobulin Variable Region/genetics; Amino Acid Sequence; Animals; Antibodies, Monoclonal/genetics; Antibody Affinity; Bacterial Proteins/chemistry; Base Sequence; Binding Sites; Biotin/metabolism; DNA; Female; Immunoglobulin Heavy Chains/chemistry; Immunoglobulin Variable Region/chemistry; Mice; Molecular Sequence Data; Sequence Alignment; Streptavidin

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Collection: 01-internacional Database: MEDLINE Main subject: Biotin / Immunoglobulin Variable Region / Avidin / Immunoglobulin Heavy Chains / Antibodies, Monoclonal Type of study: Prognostic_studies Limits: Animals Language: En Journal: FEBS Lett Year: 1993 Document type: Article Affiliation country: Israel Country of publication: United kingdom

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