Overexpression of human N-myristoyltransferase utilizing a T7 polymerase gene expression system.

Raju, R V; Datla, R S; Sharma, R K

Raju, R V; Datla, R S; Sharma, R K.

Affiliation

Raju RV; Department of Pathology, College of Medicine, Royal University Hospital, University of Saskatchewan, Saskatoon, Canada.

Protein Expr Purif ; 7(4): 431-7, 1996 Jun.

Article in En | MEDLINE | ID: mdl-8776763

Subject(s)

Acyltransferases/biosynthesis; Gene Expression Regulation, Enzymologic; Protein Processing, Post-Translational/genetics; Acyltransferases/genetics; Acyltransferases/immunology; Acyltransferases/isolation & purification; Animals; Antibody Specificity; Chromatography, Agarose; DNA-Directed RNA Polymerases/genetics; Electrophoresis, Polyacrylamide Gel; Escherichia coli/enzymology; Escherichia coli/genetics; Humans; Immune Sera/biosynthesis; Immune Sera/immunology; Immunoblotting; Plasmids; Rabbits; Reproducibility of Results; Sodium Dodecyl Sulfate/chemistry; Surface-Active Agents/chemistry; Thiogalactosides/metabolism

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Collection: 01-internacional Database: MEDLINE Main subject: Acyltransferases / Gene Expression Regulation, Enzymologic / Protein Processing, Post-Translational Limits: Animals / Humans Language: En Journal: Protein Expr Purif Journal subject: BIOLOGIA MOLECULAR Year: 1996 Document type: Article Affiliation country: Canada Country of publication: United States

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