Increased eosinophil granule proteins in gut lavage fluid from patients with inflammatory bowel disease.
Mayo Clin Proc
; 72(2): 117-23, 1997 Feb.
Article
in En
| MEDLINE
| ID: mdl-9033543
OBJECTIVE: To explore the potential role of eosinophils in the pathogenesis of inflammatory bowel disease (IBD). DESIGN: We measured the concentrations of eosinophil granule proteins-namely, major basic protein, eosinophil peroxidase, eosinophil cationic protein, and eosinophil-derived neurotoxin-in gut lavage fluid. MATERIAL AND METHODS: Ten healthy persons and 17 patients with IBD (9 with Crohn's disease and 8 with ulcerative colitis) underwent gut lavage. Each study subject submitted an early specimen when lavage effluent became liquid and a late specimen when the output became clear. The concentrations of the granule proteins were measured by immunoassay. RESULTS: The median concentrations of eosinophil-derived neurotoxin and eosinophil cationic protein were significantly higher in patients with IBD than in control subjects for both early and late lavage specimens. Excretion of eosinophil peroxidase was also significantly higher in patients with IBD than in the healthy control subjects, but only in the early specimens. No differences were noted in the concentrations of any of the proteins between patients with ulcerative colitis and those with Crohn's disease. CONCLUSION: Concentrations of eosinophil granule proteins were increased in whole gut lavage fluid from patients with IBD in comparison with healthy control subjects. These results encourage further studies of the role of eosinophils in the pathogenesis of IBD.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Peroxidases
/
Ribonucleases
/
Blood Proteins
/
Inflammatory Bowel Diseases
/
Inflammation Mediators
/
Eosinophils
/
Intestines
/
Neurotoxins
Type of study:
Observational_studies
Limits:
Adult
/
Aged
/
Female
/
Humans
/
Male
/
Middle aged
Language:
En
Journal:
Mayo Clin Proc
Year:
1997
Document type:
Article
Affiliation country:
United States
Country of publication:
United kingdom