Molecular evolution by staggered extension process (StEP) in vitro recombination.

Zhao, H; Giver, L; Shao, Z; Affholter, J A; Arnold, F H

Zhao, H; Giver, L; Shao, Z; Affholter, J A; Arnold, F H.

Affiliation

Zhao H; Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena 91125, USA.

Nat Biotechnol ; 16(3): 258-61, 1998 Mar.

Article in En | MEDLINE | ID: mdl-9528005

Subject(s)

Evolution, Molecular; Mutagenesis; Protein Engineering/methods; Recombinant Proteins/genetics; Subtilisins/genetics; Bacillus subtilis/chemistry; Gene Library; Mutation; Polymerase Chain Reaction/methods; Recombinant Proteins/metabolism; Recombination, Genetic; Sequence Analysis, DNA; Subtilisins/metabolism; Templates, Genetic

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Collection: 01-internacional Database: MEDLINE Main subject: Recombinant Proteins / Subtilisins / Protein Engineering / Mutagenesis / Evolution, Molecular Language: En Journal: Nat Biotechnol Journal subject: BIOTECNOLOGIA Year: 1998 Document type: Article Affiliation country: United States Country of publication: United States

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