Recombinant acyl-CoA:cholesterol acyltransferase-1 (ACAT-1) purified to essential homogeneity utilizes cholesterol in mixed micelles or in vesicles in a highly cooperative manner.

Chang, C C; Lee, C Y; Chang, E T; Cruz, J C; Levesque, M C; Chang, T Y

Chang, C C; Lee, C Y; Chang, E T; Cruz, J C; Levesque, M C; Chang, T Y.

Affiliation

Chang CC; Dartmouth Medical School, Department of Biochemistry, Hanover, New Hampshire 03755, USA. Ta.Yuan.Changt@Dartmouth.Edu

J Biol Chem ; 273(52): 35132-41, 1998 Dec 25.

Article in En | MEDLINE | ID: mdl-9857049

Subject(s)

Cholesterol/metabolism; Micelles; Sterol O-Acyltransferase/metabolism; Acyl Coenzyme A/metabolism; Allosteric Regulation; Bile Acids and Salts; Cholesterol Esters/biosynthesis; Chromatography, Affinity; Detergents; Humans; Phosphatidylcholines; Recombinant Proteins/isolation & purification; Recombinant Proteins/metabolism; Solubility; Sterol O-Acyltransferase/genetics; Sterol O-Acyltransferase/isolation & purification; Subcellular Fractions/enzymology

Search on Google

Add to My VHL

XML

PubMed Links

Collection: 01-internacional Database: MEDLINE Main subject: Cholesterol / Sterol O-Acyltransferase / Micelles Limits: Humans Language: En Journal: J Biol Chem Year: 1998 Document type: Article Affiliation country: United States Country of publication: United States

Search on Google

Add to My VHL

XML

PubMed Links