Molecular analysis of Salmonella entriditis isolates from the Caribbean pulsed-field gel electrophoresis
Rev Panam salud publica/Pan Am J Public Health
; 8(5): 342-7, Nov. 2000. ilus, tab
Article
in English
| MedCarib
| ID: med-118
Responsible library:
JM3.1
Localization: JM3.1; RA421.P22
ABSTRACT
Using pulsed-field electrophoresis (PFGE), between 1987 and 1996 we analyzed Salmonella enteriditis isolates from gastroenteritis cases in four Caribbean countries Barbados, Saint Kitts and Nevis, Saint Lucia, and Trinidad and Tobago. We also determined the resistance of the isolates to 12 antimicrobial agents. Of the 129 isolates of S. enteritidis available for testing, DNA digested by XbaI revealed 13 distinctive PFGE patterns. The most prevalent XbaI PFGE patterns were group 1 (88 of 129 isolates, 68.2 percent) and group 2 (26 of 129, 20.2 percent). The patterns found among S. enteritidis isolates correlated with the geographical origin of the isolates. Of the 28 isolates from Barbados, 20 of them (71.4 percent) belonged to XbaI PFGE group 2, and of the 93 isolates from Trinidad and Tobago, 78 of them (83.9 percent) belonged to group 1. SpeI digestion of S. enteritidis genome was not as discriminatory as XbaI. Overall, of the 129 isolates, 67 of them (51.9 percent) exhibited resistance to one or more of the twelve antimicrobial agents that we tested. The prevalence of resistance was 53.8 percent for the S. enteritidis isolates tested from Trinidad and Tobago, 50.0 percent for those from Barbados, 28.6 percent for those from Saint Lucia, 100.0 percent for those from the island of Saint Kitts. Resistance was highest to triple sulfur (59 of 129, 7.8 percent), ampicillin (7 of 129, 5.4 percent), and carbamycin (5 of 129, 3.9 percent). (AU)
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Collection:
International databases
Health context:
Neglected Diseases
Health problem:
Neglected Diseases
/
Zoonoses
Database:
MedCarib
Main subject:
Salmonella enteritidis
/
DNA, Bacterial
/
Electrophoresis, Gel, Pulsed-Field
Type of study:
Risk factors
Country/Region as subject:
Barbados
/
English Caribbean
/
Saint Kitts and Nevis
/
Saint Lucia
/
Trinidad and Tobago
Language:
English
Journal:
Pan Am J Public Health
Year:
2000
Document type:
Article