Prospective evaluation of enzyme-linked immunosorbent assay and immunoblot methods for the diagnosis of endemic Strongyloides stercoralis infection
Am J Trop Med Hyg
; 51(2): 175-9, Aug. 1994.
Article
in English
| MedCarib
| ID: med-2098
Responsible library:
JM3.1
Localization: JM3.1; RC960.A4
ABSTRACT
Recently described enzyme-linked immunosorbent assay (ELISA) and immunoblot methods for the detection of serum IgG against Strongyloides stercoralis larval antigens were prospectively evaluated for the diagnosis of endemic strongyloidiasis. A modification of the ELISA involved preincubation of sera with Onchocerca gutturosa phosphate-buffered saline-soluble extract to remove cross-reactivity with other helminths. The sensitivity of the ELISA increased from 80 percent and 85 percent following preincubation. Similarly, there was an increase in specifity from 94 percent to 97 percent. The IgG recognition of 41-, 31-, and 28-kD filariform larval components showed sensitivities of 100 percent, 85 percent and 65 percent, respectively. Both the ELISA following incubation of sera with O. gutturosa extract and serum IgG reactivity to a 41-kD larval component using immunoblotting are sensitive and specific techniques for diagnosing endemic strongyloidiasis.(AU)
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Collection:
International databases
Health context:
Neglected Diseases
Health problem:
Helminthiasis
/
Neglected Diseases
/
Onchocerciasis
/
Zoonoses
Database:
MedCarib
Main subject:
Strongyloidiasis
/
Enzyme-Linked Immunosorbent Assay
/
Antibodies, Helminth
/
Immunoblotting
/
Strongyloides stercoralis
Type of study:
Diagnostic study
/
Observational study
Language:
English
Journal:
Am J Trop Med Hyg
Year:
1994
Document type:
Article