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SARS-CoV-2 detection from nasopharyngeal swab samples without RNA extraction
Carolina Beltran-Pavez; Chantal L. Marquez; Gabriela Munoz; Fernando Valiente-Echeverria; Aldo Gaggero; Ricardo Soto-Rifo; Gonzalo P. Barriga.
Affiliation
  • Carolina Beltran-Pavez; Universidad de Chile Faculty of Medicine
  • Chantal L. Marquez; Universidad de Chile
  • Gabriela Munoz; Hospital Clinico de la Universidad de Chile
  • Fernando Valiente-Echeverria; Universidad de Chile Faculty of Medicine
  • Aldo Gaggero; Universidad de Chile Faculty of Medicine
  • Ricardo Soto-Rifo; Universidad de Chile Faculty of Medicine
  • Gonzalo P. Barriga; Universidad de Chile Faculty of Medicine
Preprint in English | bioRxiv | ID: ppbiorxiv-013508
ABSTRACT
The ongoing COVID-19 pandemic has reached more than 200 countries and territories worldwide. Given the large requirement of SARS-CoV-2 diagnosis and considering that RNA extraction kits are in short supply, we investigated whether two commercial RT-qPCR kits were compatible with direct SARS-CoV-2 detection from nasopharyngeal swab samples. We show that one of the tested kits is fully compatible with direct SARS-CoV-2 detection suggesting that omission of an RNA extraction step should be considered in SARS-CoV-2 diagnosis.
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Full text: Available Collection: Preprints Database: bioRxiv Language: English Year: 2020 Document type: Preprint
Full text: Available Collection: Preprints Database: bioRxiv Language: English Year: 2020 Document type: Preprint
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