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SARS-CoV-2 Productively Infects Human Gut Enterocytes
Mart M. Lamers; Joep Beumer; Jelte van der Vaart; Kèvin Knoops; Jens Puschhof; Tim I. Breugem; Raimond B.G. Ravelli; J. Paul van Schayck; Anna Z. Mykytyn; Hans Q. Duimel; Elly van Donselaar; Samra Riesebosch; Helma J.H. Kuijpers; Debby Schipper; Willine J. van de Wetering; Miranda de Graaf; Marion Koopmans; Edwin Cuppen; Peter J. Peters; Bart L. Haagmans; Hans Clevers.
Affiliation
  • Mart M. Lamers; Viroscience Department, Erasmus Medical Center, Rotterdam, Netherlands.
  • Joep Beumer; Oncode Institute, Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Center, Uppsalalaan 8, Utrecht, 3584 CT, Netherlands
  • Jelte van der Vaart; Oncode Institute, Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Center, Uppsalalaan 8, Utrecht, 3584 CT, Netherlands
  • Kèvin Knoops; The Maastricht Multimodal Molecular Imaging Institute, Maastricht University, 6229 ER Maastricht, Netherlands.
  • Jens Puschhof; Oncode Institute, Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Center, Uppsalalaan 8, Utrecht, 3584 CT, Netherlands
  • Tim I. Breugem; Viroscience Department, Erasmus Medical Center, Rotterdam, Netherlands.
  • Raimond B.G. Ravelli; The Maastricht Multimodal Molecular Imaging Institute, Maastricht University, 6229 ER Maastricht, Netherlands.
  • J. Paul van Schayck; The Maastricht Multimodal Molecular Imaging Institute, Maastricht University, 6229 ER Maastricht, Netherlands.
  • Anna Z. Mykytyn; Viroscience Department, Erasmus Medical Center, Rotterdam, Netherlands.
  • Hans Q. Duimel; The Maastricht Multimodal Molecular Imaging Institute, Maastricht University, 6229 ER Maastricht, Netherlands.
  • Elly van Donselaar; The Maastricht Multimodal Molecular Imaging Institute, Maastricht University, 6229 ER Maastricht, Netherlands.
  • Samra Riesebosch; Viroscience Department, Erasmus Medical Center, Rotterdam, Netherlands.
  • Helma J.H. Kuijpers; The Maastricht Multimodal Molecular Imaging Institute, Maastricht University, 6229 ER Maastricht, Netherlands.
  • Debby Schipper; Viroscience Department, Erasmus Medical Center, Rotterdam, Netherlands.
  • Willine J. van de Wetering; The Maastricht Multimodal Molecular Imaging Institute, Maastricht University, 6229 ER Maastricht, Netherlands.
  • Miranda de Graaf; Viroscience Department, Erasmus Medical Center, Rotterdam, Netherlands.
  • Marion Koopmans; Viroscience Department, Erasmus Medical Center, Rotterdam, Netherlands.
  • Edwin Cuppen; Center for Molecular Medicine and Oncode Institute, University Medical Centre Utrecht, Utrecht, Netherlands; Hartwig Medical Foundation, Amsterdam, Netherlands.
  • Peter J. Peters; The Maastricht Multimodal Molecular Imaging Institute, Maastricht University, 6229 ER Maastricht, Netherlands.
  • Bart L. Haagmans; Viroscience Department, Erasmus Medical Center, Rotterdam, Netherlands.
  • Hans Clevers; Oncode Institute, Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Center, Uppsalalaan 8, Utrecht, 3584 CT, Netherlands
Preprint in English | bioRxiv | ID: ppbiorxiv-060350
Journal article
A scientific journal published article is available and is probably based on this preprint. It has been identified through a machine matching algorithm, human confirmation is still pending.
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ABSTRACT
COVID-19, caused by SARS-CoV-2, is an influenza-like disease with a respiratory route of transmission, yet clinical evidence suggests that the intestine may present another viral target organ. Indeed, the SARS-CoV-2 receptor angiotensin converting enzyme 2 (ACE2) is highly expressed on differentiated enterocytes. In human small intestinal organoids, enterocytes were readily infected by SARS-CoV and SARS-CoV-2 as demonstrated by confocal- and electron-microscopy. Consequently, significant titers of infectious viral particles were measured. mRNA expression analysis revealed strong induction of a generic viral response program. We conclude that intestinal epithelium supports SARS-CoV-2 replication. One Sentence SummarySARS-CoV-2 infection of enterocytes in human small intestinal organoids
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Full text: Available Collection: Preprints Database: bioRxiv Type of study: Prognostic study Language: English Year: 2020 Document type: Preprint
Fulltext
Add to My VHL
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Full text: Available Collection: Preprints Database: bioRxiv Type of study: Prognostic study Language: English Year: 2020 Document type: Preprint