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Zebrafish studies on the vaccine candidate to COVID-19, the Spike protein: Production of antibody and adverse reaction
Bianca H Ventura Fernandes; Natalia Martins Feitosa; Ana Paula Barbosa; Camila Gasque Bomfim; Anali MB Garnique; Francisco IF Gomes; Rafael T Nakajima; Marco AA Belo; Silas Fernandes Eto; Dayanne Carla Fernandes; Guilherme Malafaia; Wilson G Manrique; Gabriel Conde; Roberta RC Rosales; Iris Todeschini; Ilo Rivero; Edgar Llontop; German G Sgro; Gabriel Umaji Oka; Natalia Fernanda Bueno; Fausto K Ferraris; Mariana TQ de Magalhaes; Renata J Medeiros; Juliana MM Gomes; Mara Souza Junqueira; Katia Conceicao; Leticia G. Pontes; Antonio Condino Neto; Andrea C Perez; Leonardo G Barcellos; Jose Dias Correa Junior; Erick Gustavo Dorlass; Niels OS Camara; Edison Luiz Durigon; Fernando Q Cunha; Rafael H Nobrega; Glaucia M Machado-Santelli; Chuck S Farah; Flavio P Veras; Jorge Galindo-Villegas; Leticia Costa-Lotufo; Thiago M Cunha; Roger Chammas; Luciani R. Carvalho; Cristiane R. Guzzo; Ives Charlie-Silva.
Affiliation
  • Bianca H Ventura Fernandes; Laboratorio de Controle Genetico e Sanitario, Diretoria Tecnica de Apoio ao Ensino e Pesquisa, Faculdade de Medicina da Universidade de Sao Paulo
  • Natalia Martins Feitosa; Integrated Laboratory of Translational Bioscience (LIBT), Institute of Biodiversity and Sustainability (NUPEM), Federal University of Rio de Janeiro (UFRJ)- Mac
  • Ana Paula Barbosa; Department of Microbiology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil.
  • Camila Gasque Bomfim; Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, Sao Paulo, SP, Brazil, Departamento de Microbiologia, Instituto de Ciencias Biomedi
  • Anali MB Garnique; Department of Cell Biology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil.
  • Francisco IF Gomes; Department of Pharmacology, Center of Research in Inflammatory Diseases, Ribeirao Preto Medical School, University of Sao Paulo, Ribeirao Preto, Sao Paulo, Braz
  • Rafael T Nakajima; Reproductive and Molecular Biology Group, Department of Morphology, Institute of Biosciences, Sao Paulo State University, Botucatu, Sao Paulo, Brazil.
  • Marco AA Belo; Department of Preventive Veterinary Medicine, Sao Paulo State University, Jaboticabal, Sao Paulo, Brazil / Laboratory of Animal Pharmacology and Toxicology, Br
  • Silas Fernandes Eto; Postgraduate Program in Health Sciences, PROCISA, Federal University of Roraima, Brazil.
  • Dayanne Carla Fernandes; Immunochemistry Laboratory, Butantan Institute, Sao Paulo, Brazil.
  • Guilherme Malafaia; Biological Research Laboratory, Goiano Federal Institute, Urutai Campus, GO, Brazil.
  • Wilson G Manrique; Aquaculture Health Research and Extension Group, GRUPESA, Aquaculture Health Laboratory, LABSA, Department of Veterinary Medicine, Federal University of Rondoni
  • Gabriel Conde; Department of Preventive Veterinary Medicine, Sao Paulo State University, Jaboticabal, Sao Paulo, Brazil.
  • Roberta RC Rosales; Department of Cell and Molecular Biology, Ribeirao Preto Medical School, University of Sao Paulo, Ribeirao Preto, Sao Paulo, Brazil.
  • Iris Todeschini; Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, Brazil.
  • Ilo Rivero; Pontificia Universidade Catolica de Minas Gerais
  • Edgar Llontop; Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, Brazil.
  • German G Sgro; Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, Sao Paulo, SP, Brazil, Departamento de Ciencias Biomoleculares, Faculdade de Cienc
  • Gabriel Umaji Oka; Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, Brazil.
  • Natalia Fernanda Bueno; Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, Sao Paulo, SP, Brazil; Departamento de Microbiologia, Instituto de Ciencias Biomedi
  • Fausto K Ferraris; Department of Pharmacology and Toxicology, Oswaldo Cruz Foundation, FIOCRUZ, Rio de Janeiro, Brazil.
  • Mariana TQ de Magalhaes; Department of Biochemistry and Immunology, Institute of Biological Sciences , Federal University of Minas Gerais, Belo Horizonte, Brazil.
  • Renata J Medeiros; Laboratory of Physiology, Zebrafish INCQS, Fiocruz Facility, Department of Pharmacology and Toxicology, DFT, INCQS, National Institute for Quality Control in
  • Juliana MM Gomes; Transplantation Immunobiology Lab, Department of Immunology, Institute of Biomedical Sciences, Universidade de Sao Paulo, Brazil.
  • Mara Souza Junqueira; Center for Translational Research in Oncology, Cancer Institute of the State of Sao Paulo, Faculty of Medicine, University of Sao Paulo, Sao Paulo, Brazil.
  • Katia Conceicao; Laboratory of Peptide Biochemistry, Federal University of Sao Paulo, Brazil.
  • Leticia G. Pontes; Laboratory of Human Immunology, Department Immunology, Institute Biomedical Sciences, University Sao Paulo, Sao Paulo, Brazil.
  • Antonio Condino Neto; Laboratory of Human Immunology, Department Immunology, Institute Biomedical Sciences, University Sao Paulo, Sao Paulo, Brazil.
  • Andrea C Perez; Department of Pharmacology, Universidade Federal de Minas Gerais, Brazil.
  • Leonardo G Barcellos; Graduate Program of Pharmacology, Federal University of Santa Maria, Brazil; Laboratory of Fish Physiology, Graduate Program of Bioexperimentation and of Enviro
  • Jose Dias Correa Junior; Laboratorio do Estudo da Interacao Quimico Biologica e da Reproducao Animal, LIQBRA, Bloco O3,174, Departamento de Morfologia Instituto de Ciencias Biologicas,
  • Erick Gustavo Dorlass; Department of Microbiology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil.
  • Niels OS Camara; Transplantation Immunobiology Lab, Department of Immunology, Institute of Biomedical Sciences, Universidade de Sao Paulo, Brazil.
  • Edison Luiz Durigon; Department of Microbiology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil.
  • Fernando Q Cunha; Department of Pharmacology, Center of Research in Inflammatory Diseases, Ribeirao Preto Medical School, University of Sao Paulo, Ribeirao Preto, Sao Paulo, Braz
  • Rafael H Nobrega; Reproductive and Molecular Biology Group, Department of Morphology, Institute of Biosciences, Sao Paulo State University, Botucatu, Sao Paulo, Brazil.
  • Glaucia M Machado-Santelli; Department of Cell Biology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil.
  • Chuck S Farah; Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, Brazil.
  • Flavio P Veras; Center of Research in Inflammatory Diseases, Ribeirao Preto Medical School, University of Sao Paulo, Ribeirao Preto, Sao Paulo, Brazil; Department of Pharmacolo
  • Jorge Galindo-Villegas; Faculty of Biosciences and Aquaculture, Nord University, 8049 Bodo, Norway
  • Leticia Costa-Lotufo; Department of Pharmacology, Institute of Biomedical Sciences, Universidade de Sao Paulo, Brazil.
  • Thiago M Cunha; Center of Research in Inflammatory Diseases, Ribeirao Preto Medical School, University of Sao Paulo, Ribeirao Preto, Sao Paulo, Brazil; Department of Pharmacolo
  • Roger Chammas; Laboratorio de Controle Genetico e Sanitario, Diretoria Tecnica de Apoio ao Ensino e Pesquisa, Faculdade de Medicina da Universidade de Sao Paulo; Centro de Inv
  • Luciani R. Carvalho; Laboratorio de Controle Genetico e Sanitario, Diretoria Tecnica de Apoio ao Ensino e Pesquisa, Faculdade de Medicina da Universidade de Sao Paulo; Disciplina de
  • Cristiane R. Guzzo; Department of Microbiology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil.
  • Ives Charlie-Silva; Department of Pharmacology, Institute of Biomedical Sciences, Universidade de Sao Paulo, Brazil.
Preprint in English | bioRxiv | ID: ppbiorxiv-346262
ABSTRACT
Establishing new experimental animal models to assess the safety and immune response to the antigen used in the development of COVID-19 vaccine is an imperative issue. Based on the advantages of using zebrafish as a model in research, herein we suggest doing this to test the safety of the putative vaccine candidates and to study immune response against the virus. We produced a recombinant N-terminal fraction of the Spike SARS-CoV-2 protein and injected it into adult female zebrafish. The specimens generated humoral immunity and passed the antibodies to the eggs. However, they presented adverse reactions and inflammatory responses similar to severe cases of human COVID-19. The analysis of the structure and function of zebrafish and human Angiotensin-converting enzyme 2, the main human receptor for virus infection, presented remarkable sequence similarities. Moreover, bioinformatic analysis predicted protein-protein interaction of the Spike SARS-CoV-2 fragment and the Toll-like receptor pathway. It might help in the choice of future therapeutic pharmaceutical drugs to be studied. Based on the in vivo and in silico results presented here, we propose the zebrafish as a model for translational research into the safety of the vaccine and the immune response of the vertebrate organism to the SARS-CoV-2 virus.
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Full text: Available Collection: Preprints Database: bioRxiv Type of study: Prognostic study Language: English Year: 2020 Document type: Preprint
Fulltext
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Full text: Available Collection: Preprints Database: bioRxiv Type of study: Prognostic study Language: English Year: 2020 Document type: Preprint