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Potent SARS-CoV-2 neutralizing antibodies selected from a human antibody library constructed decades ago
Min Qiang; Peixiang Ma; Yu Li; Hejun Liu; Adam Harding; Chenyu Min; Lili Liu; Meng Yuan; Qun Ji; Pingdong Tao; Xiaojie Shi; Zhean Li; Fulian Wang; Yu Zhang; Nicholas C. Wu; Chang-Chun D. Lee; Xueyong Zhu; Javier Gilbert-Jaramillo; Abhishek Saxena; Xingxu Huang; Hou Wang; William James; Raymond A. Dwek; Ian A. Wilson; Guang Yang; Richard A. Lerner.
Affiliation
  • Min Qiang; ShanghaiTech University
  • Peixiang Ma; ShanghaiTech University
  • Yu Li; ShanghaiTech University; Chinese Academy of Sciences; University of Chinese Academy of Sciences
  • Hejun Liu; The Scripps Research Institute
  • Adam Harding; University of Oxford
  • Chenyu Min; Velox Pharmaceuticals
  • Lili Liu; ShanghaiTech University
  • Meng Yuan; The Scripps Research Institute
  • Qun Ji; ShanghaiTech University
  • Pingdong Tao; ShanghaiTech University; Chinese Academy of Sciences; University of Chinese Academy of Sciences
  • Xiaojie Shi; ShanghaiTech University
  • Zhean Li; ShanghaiTech University
  • Fulian Wang; ShanghaiTech University; Chinese Academy of Sciences; University of Chinese Academy of Sciences
  • Yu Zhang; ShanghaiTech University
  • Nicholas C. Wu; The Scripps Research Institute
  • Chang-Chun D. Lee; The Scripps Research Institute
  • Xueyong Zhu; The Scripps Research Institute
  • Javier Gilbert-Jaramillo; University of Oxford
  • Abhishek Saxena; ShanghaiTech University
  • Xingxu Huang; ShanghaiTech University
  • Hou Wang; ShOx Science Limited
  • William James; University of Oxford
  • Raymond A. Dwek; Oxford Glycobiology Institute
  • Ian A. Wilson; The Scripps Research Institute
  • Guang Yang; ShanghaiTech University;Velox Pharmaceuticals
  • Richard A. Lerner; The Scripps Research Institute
Preprint in English | bioRxiv | ID: ppbiorxiv-370676
ABSTRACT
Combinatorial antibody libraries not only effectively reduce antibody discovery to a numbers game, but enable documentation of the history of antibody responses in an individual. The SARS-CoV-2 pandemic has prompted a wider application of this technology to meet the public health challenge of pandemic threats in the modern era. Herein, we used a combinatorial human antibody library constructed 20 years before the COVID-19 pandemic to discover three highly potent antibodies that selectively bind SARS-CoV-2 spike protein and neutralize authentic SARS-CoV-2 virus. Compared to neutralizing antibodies from COVID-19 patients with generally low somatic hypermutation (SHM), these antibodies contain over 13-22 SHMs, many of which are involved in specific interactions in crystal structures with SARS-CoV-2 spike RBD. The identification of these somatically mutated antibodies in a pre-pandemic library raises intriguing questions about the origin and evolution of human immune responses to SARS-CoV-2.
License
cc_by_nc_nd
Full text: Available Collection: Preprints Database: bioRxiv Type of study: Prognostic study Language: English Year: 2020 Document type: Preprint
Full text: Available Collection: Preprints Database: bioRxiv Type of study: Prognostic study Language: English Year: 2020 Document type: Preprint
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