Your browser doesn't support javascript.
loading
Multi-omic profiling reveals widespread dysregulation of innate immunity and hematopoiesis in COVID-19
Aaron J. Wilk; Madeline J. Lee; Bei Wei; Benjamin Parks; Ruoxi Pi; Giovanny J. Martinez-Colon; Thanmayi Ranganath; Nancy Q. Zhao; Shalina Taylor; Winston Becker; - Stanford COVID-19 Biobank; David Jimenez-Morales; Andra L. Blomkalns; Euan A. Ashley; Kari C. Nadeau; Samuel Yang; Susan Holmes; Marlene Rabinovitch; Angela J. Rogers; William J. Greenleaf; Catherine A. Blish.
Affiliation
  • Aaron J. Wilk; Stanford University School of Medicine
  • Madeline J. Lee; Stanford University School of Medicine
  • Bei Wei; Stanford University School of Medicine
  • Benjamin Parks; Stanford University School of Medicine
  • Ruoxi Pi; Stanford University School of Medicine
  • Giovanny J. Martinez-Colon; Stanford University School of Medicine
  • Thanmayi Ranganath; Stanford University School of Medicine
  • Nancy Q. Zhao; Stanford University School of Medicine
  • Shalina Taylor; Stanford University School of Medicine
  • Winston Becker; Stanford University School of Medicine
  • - Stanford COVID-19 Biobank; -
  • David Jimenez-Morales; Stanford University School of Medicine
  • Andra L. Blomkalns; Stanford University School of Medicine
  • Euan A. Ashley; Stanford University School of Medicine
  • Kari C. Nadeau; Stanford University School of Medicine
  • Samuel Yang; Stanford University School of Medicine
  • Susan Holmes; Stanford University
  • Marlene Rabinovitch; Stanford University School of Medicine
  • Angela J. Rogers; Stanford University School of Medicine
  • William J. Greenleaf; Stanford University School of Medicine
  • Catherine A. Blish; Stanford University School of Medicine
Preprint in English | bioRxiv | ID: ppbiorxiv-423363
ABSTRACT
Our understanding of protective vs. pathologic immune responses to SARS-CoV-2, the virus that causes Coronavirus disease 2019 (COVID-19), is limited by inadequate profiling of patients at the extremes of the disease severity spectrum. Here, we performed multi-omic single-cell immune profiling of 64 COVID-19 patients across the full range of disease severity, from outpatients with mild disease to fatal cases. Our transcriptomic, epigenomic, and proteomic analyses reveal widespread dysfunction of peripheral innate immunity in severe and fatal COVID-19, with the most profound disturbances including a prominent neutrophil hyperactivation signature and monocytes with anti-inflammatory features. We further demonstrate that emergency myelopoiesis is a prominent feature of fatal COVID-19. Collectively, our results reveal disease severity-associated immune phenotypes in COVID-19 and identify pathogenesis-associated pathways that are potential targets for therapeutic intervention. One Sentence SummarySingle-cell profiling demonstrates multifarious dysregulation of innate immune phenotype associated with COVID-19 severity.
License
cc_no
Full text: Available Collection: Preprints Database: bioRxiv Type of study: Prognostic study Language: English Year: 2020 Document type: Preprint
Full text: Available Collection: Preprints Database: bioRxiv Type of study: Prognostic study Language: English Year: 2020 Document type: Preprint
...