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Design and clinical validation of a 3D-printed nasopharyngeal swab for COVID-19 testing
Joshua K Tay; Gail B Cross; Chun Kiat Lee; Benedict Yan; Jerold Loh; Zhen Yu Lim; Nicholas Ngiam; Jeremy Chee; Soo Wah Gan; Anmol Saraf; Wai Tung Eason Chow; Han Lee Goh; Chor Hiang Siow; Derrick WQ Lian; Woei Shyang Loh; Kwok Seng Loh; Vincent TK Chow; De Yun Wang; Jerry YH Fuh; Ching-Chiuan Yen; John EL Wong; David M Allen.
Affiliation
  • Joshua K Tay; National University of Singapore
  • Gail B Cross; National University of Singapore
  • Chun Kiat Lee; National University Hospital Singapore
  • Benedict Yan; National University Hospital Singapore
  • Jerold Loh; National University Hospital Singapore
  • Zhen Yu Lim; National University Hospital Singapore
  • Nicholas Ngiam; National University Hospital Singapore
  • Jeremy Chee; National University Hospital Singapore
  • Soo Wah Gan; National University of Singapore
  • Anmol Saraf; National University of Singapore
  • Wai Tung Eason Chow; National University of Singapore
  • Han Lee Goh; National University of Singapore
  • Chor Hiang Siow; National University of Singapore
  • Derrick WQ Lian; National University of Singapore
  • Woei Shyang Loh; National University of Singapore
  • Kwok Seng Loh; National University of Singapore
  • Vincent TK Chow; National University of Singapore
  • De Yun Wang; National University of Singapore
  • Jerry YH Fuh; National University of Singapore
  • Ching-Chiuan Yen; National University of Singapore
  • John EL Wong; National University of Singapore
  • David M Allen; National University of Singapore
Preprint in En | PREPRINT-MEDRXIV | ID: ppmedrxiv-20134791
ABSTRACT
We describe the development and validation of a novel 3D-printed nasopharyngeal swab for the identification of SARS-CoV-2. We subjected the novel swab to mechanical and fluid absorption testing ex-vivo, and confirmed its ability to retain and release murine coronavirus and SARS-CoV-2. Compared to the Copan FLOQSwab, the novel swab displayed excellent correlation of RT-PCR cycle threshold values on paired clinical testing in COVID-19 patients, at r = 0.918 and 0.943 for the SARS-CoV-2 ORF1/a and sarbecovirus E-gene respectively. Overall positive and negative percent agreement was 90.6% and 100% respectively on a dual-assay RT-PCR platform, with discordant samples observed only at high cycle thresholds. When carefully designed and tested, 3D-printed swabs are a viable alternative to traditional swabs and will help mitigate strained resources in the escalating COVID-19 pandemic.
License
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Full text: 1 Collection: 09-preprints Database: PREPRINT-MEDRXIV Type of study: Prognostic_studies Language: En Year: 2020 Document type: Preprint
Full text: 1 Collection: 09-preprints Database: PREPRINT-MEDRXIV Type of study: Prognostic_studies Language: En Year: 2020 Document type: Preprint