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A Sensitive, Rapid, and Portable CasRx-based Diagnostic Assay for SARS-CoV2
Daniel J Brogan; Duverney Chaverra-Rodriguez; Calvin P Lin; Andrea L Smidler; Ting Yang; Lenissa M Alcantara; Igor Antoshechkin; Junru Liu; Robyn R Raban; Pedro Belda-ferre; Rob Knight; Elizabeth A Komives; Omar S. Akbari.
Affiliation
  • Daniel J Brogan; UCSD
  • Duverney Chaverra-Rodriguez; UCSD
  • Calvin P Lin; UCSD
  • Andrea L Smidler; UCSD
  • Ting Yang; UCSD
  • Lenissa M Alcantara; UCSD
  • Igor Antoshechkin; Caltech
  • Junru Liu; UCSD
  • Robyn R Raban; UCSD
  • Pedro Belda-ferre; UCSD
  • Rob Knight; UCSD
  • Elizabeth A Komives; Ucsd
  • Omar S. Akbari; UCSD
Preprint in English | medRxiv | ID: ppmedrxiv-20212795
Journal article
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ABSTRACT
Since its first emergence from China in late 2019, the SARS-CoV-2 virus has spread globally despite unprecedented containment efforts, resulting in a catastrophic worldwide pandemic. Successful identification and isolation of infected individuals can drastically curtail virus spread and limit outbreaks. However, during the early stages of global transmission, point-of-care diagnostics were largely unavailable and continue to remain difficult to procure, greatly inhibiting public health efforts to mitigate spread. Furthermore, the most prevalent testing kits rely on reagent- and time-intensive protocols to detect viral RNA, preventing rapid and cost-effective diagnosis. Therefore the development of an extensive toolkit for point-of-care diagnostics that is expeditiously adaptable to new emerging pathogens is of critical public health importance. Recently, a number of novel CRISPR-based diagnostics have been developed to detect COVID-19. Herein, we outline the development of a CRISPR-based nucleic acid molecular diagnostic utilizing a Cas13d ribonuclease derived from Ruminococcus flavefaciens (CasRx) to detect SARS-CoV-2, an approach we term SENSR (Sensitive Enzymatic Nucleic-acid Sequence Reporter). We demonstrate SENSR robustly detects SARS-CoV-2 sequences in both synthetic and patient-derived samples by lateral flow and fluorescence, thus expanding the available point-of-care diagnostics to combat current and future pandemics.
License
cc_by_nd
Full text: Available Collection: Preprints Database: medRxiv Type of study: Diagnostic study Language: English Year: 2020 Document type: Preprint
Full text: Available Collection: Preprints Database: medRxiv Type of study: Diagnostic study Language: English Year: 2020 Document type: Preprint
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