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Integrated Single-Cell Atlases Reveal an Oral SARS-CoV-2 Infection and Transmission Axis
Kevin M Byrd; Ni Huang; Paola Perez; Takafumi Kato; Yu Mikami; Kenichi Okuda; Rodney C. Gilmore; Cecilia Dominguez Conde; Billel Gasmi; Sydney Stein; Margaret Beach; Eileen Pelayo; Jose Maldonado-Ortiz; Bernard LaFont; Ricardo Padilla; Valerie Murrah; Robert Maile; Will Lovell; Shannon Wallet; Natalie M Bowman; Suzanne L Meinig; Matthew C Wolfgang; Saibyasachi N. Choudhury; Mark Novotny; Brian D Aevermann; Richard Scheuermann; Gabrielle Cannon; Carlton Anderson; Julie Marchesan; Mandy Bush; Marcelo Freire; Adam Kimple; Daniel L Herr; Joseph Rabin; Alison Grazioli; Benjamin N. French; Thomas JF Pranzatelli; John A. Chiorini; David E. Kleiner; Stefania Pittaluga; Stephen Hewitt; Peter D. Burbelo; Daniel Chertow; - NIH COVID-19 Autopsy Consortium; - HCA Oral and Craniofacial Biological Network; Karen M Frank; Janice Lee; Richard C. Boucher C. Boucher; Sarah A. Teichmann; Blake M Warner.
Affiliation
  • Kevin M Byrd; ADA Science & Research Institute
  • Ni Huang; Wellcome Sanger Institute
  • Paola Perez; National Institutes of Health
  • Takafumi Kato; University of North Carolina at Chapel Hill
  • Yu Mikami; University of North Carolina at Chapel Hill
  • Kenichi Okuda; University of North Carolina at Chapel Hill
  • Rodney C. Gilmore; University of North Carolina at Chapel Hill
  • Cecilia Dominguez Conde; Wellcome Sanger Institute
  • Billel Gasmi; National Institutes of Health
  • Sydney Stein; National Institutes of Health
  • Margaret Beach; National Institutes of Health
  • Eileen Pelayo; National Institutes of Health
  • Jose Maldonado-Ortiz; National Institutes of Health
  • Bernard LaFont; National Institutes of Health
  • Ricardo Padilla; University of North Carolina Adams School of Dentistry
  • Valerie Murrah; University of North Carolina Adams School of Dentistry
  • Robert Maile; University of North Carolina at Chapel Hill
  • Will Lovell; University of North Carolina Adams School of Dentistry
  • Shannon Wallet; University of North Carolina at Chapel Hill
  • Natalie M Bowman; University of North Carolina at Chapel Hill
  • Suzanne L Meinig; University of North Carolina at Chapel Hill
  • Matthew C Wolfgang; University of North Carolina at Chapel Hill
  • Saibyasachi N. Choudhury; J. Craig Venter Institute
  • Mark Novotny; J. Craig Venter Institute
  • Brian D Aevermann; J. Craig Venter Institute
  • Richard Scheuermann; J. Craig Venter Institute
  • Gabrielle Cannon; University of North Carolina at Chapel Hill
  • Carlton Anderson; University of North Carolina at Chapel Hill
  • Julie Marchesan; University of North Carolina Adams School of Dentistry
  • Mandy Bush; University of North Carolina Adams School of Dentistry
  • Marcelo Freire; J. Craig Venter Institute
  • Adam Kimple; University of North Carolina at Chapel Hill
  • Daniel L Herr; University of Maryland School of Medicine
  • Joseph Rabin; University of Maryland School of Medicine
  • Alison Grazioli; National Institutes of Health
  • Benjamin N. French; National Institute of Health
  • Thomas JF Pranzatelli; National Institutes of Health
  • John A. Chiorini; National Institutes of Health
  • David E. Kleiner; National Institutes of Health
  • Stefania Pittaluga; National Institutes of Health
  • Stephen Hewitt; National Institutes of Health
  • Peter D. Burbelo; National Institutes of Health
  • Daniel Chertow; National Institutes of Health
  • - NIH COVID-19 Autopsy Consortium;
  • - HCA Oral and Craniofacial Biological Network;
  • Karen M Frank; National Institutes of Health
  • Janice Lee; National Institutes of Health
  • Richard C. Boucher C. Boucher; University of North Carolina at Chapel Hill
  • Sarah A. Teichmann; Wellcome Sanger Institute
  • Blake M Warner; National Institutes of Health
Preprint in English | medRxiv | ID: ppmedrxiv-20219089
ABSTRACT
Despite signs of infection, the involvement of the oral cavity in COVID-19 is poorly understood. To address this, single-cell RNA sequencing data-sets were integrated from human minor salivary glands and gingiva to identify 11 epithelial, 7 mesenchymal, and 15 immune cell clusters. Analysis of SARS-CoV-2 viral entry factor expression showed enrichment in epithelia including the ducts and acini of the salivary glands and the suprabasal cells of the mucosae. COVID-19 autopsy tissues confirmed in vivo SARS-CoV-2 infection in the salivary glands and mucosa. Saliva from SARS-CoV-2-infected individuals harbored epithelial cells exhibiting ACE2 expression and SARS-CoV-2 RNA. Matched nasopharyngeal and saliva samples found distinct viral shedding dynamics and viral burden in saliva correlated with COVID-19 symptoms including taste loss. Upon recovery, this cohort exhibited salivary antibodies against SARS-CoV-2 proteins. Collectively, the oral cavity represents a robust site for COVID-19 infection and implicates saliva in viral transmission.
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Full text: Available Collection: Preprints Database: medRxiv Type of study: Cohort_studies / Observational study / Prognostic study Language: English Year: 2020 Document type: Preprint
Full text: Available Collection: Preprints Database: medRxiv Type of study: Cohort_studies / Observational study / Prognostic study Language: English Year: 2020 Document type: Preprint
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