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SARS-CoV-2 genome sequencing from COVID-19 in Ecuadorian patients: a whole country analysis.
Preprint
in English
| medRxiv
| ID: ppmedrxiv-21253620
ABSTRACT
SARS-CoV-2, the etiological agent of COVID-19, was first described in Wuhan, China in December 2019 and has now spread globally. Ecuador was the second country in South America to confirm cases and Guayaquil was one of the first cities in the world to experience high mortality due to COVID-19. The aim of this study was to describe the lineages circulating throughout the country and to compare the mutations in local variants, to the reference strain. In this work we used the MinION platform (Oxford Nanopore Technologies) to sequence the whole SARS-CoV-2 genomes of 119 patients from all provinces of Ecuador, using the ARTIC network protocols. Our data from lineage assignment of the one hundred and nineteen whole genomes revealed twenty different lineages. All genomes presented differences in the S gene compared to the Wuhan reference strain, being the D614G amino acid replacement the most common change. The B.1.1.119 lineage was the most frequent and was found in several locations in the Coast and Andean region. Three sequences were assigned to the new B.1.1.7 lineage. Our work is an important contribution to the understanding of the epidemiology of SARS-CoV-2 in Ecuador and South America. HighlightsO_LIAll 119 genomes showed mutations compared to the reference strain, which could be important to understand the virulence, severity and transmissibility of the virus. C_LIO_LIUntil January 17, three sequences were assigned to the new B.1.1.7 lineage. C_LIO_LIOur findings suggest that there were at least twenty independent introductions of SARS-CoV-2 to Ecuador. C_LI Article Summary LineWe report 119 sequences of SARS-CoV-2 across all Ecuadorian provinces, 20 different lineages were found until January 17th, including B.1.1.7.
cc_by_nc_nd
Full text:
Available
Collection:
Preprints
Database:
medRxiv
Type of study:
Etiology study
/
Prognostic study
Language:
English
Year:
2021
Document type:
Preprint