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T cell response to intact SARS-CoV-2 includes coronavirus cross-reactive and variant-specific components
Lichen Jing; Xia Wu; Maxwell P Krist; Tien-Ying Hsiang; Victoria L Campbell; Christopher L McClurkan; Sydney M Favors; Lawrence A Hemingway; Charmie Godornes; Denise Q Tong; Stacy Selke; Angela C LeClair; Chu-Woo Pyo; Daniel E Geraghty; Laing J Laing; Anna Wald; Michael Gale Jr.; David M Koelle.
Affiliation
  • Lichen Jing; University of Washington
  • Xia Wu; University of Washington
  • Maxwell P Krist; University of Washington
  • Tien-Ying Hsiang; University of Washington
  • Victoria L Campbell; University of Washington
  • Christopher L McClurkan; University of Washington
  • Sydney M Favors; University of Washington
  • Lawrence A Hemingway; University of Washington
  • Charmie Godornes; University of Washington
  • Denise Q Tong; University of Washington
  • Stacy Selke; University of Washington
  • Angela C LeClair; University of Washington
  • Chu-Woo Pyo; Fred Hutchinson Cancer Research Center
  • Daniel E Geraghty; Fred Hutchinson Cancer Research Center
  • Laing J Laing; University of Washington
  • Anna Wald; University of Washington
  • Michael Gale Jr.; University of Washington
  • David M Koelle; University of Washington
Preprint in English | medRxiv | ID: ppmedrxiv-22269497
Journal article
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ABSTRACT
SARS-CoV-2 provokes a brisk T cell response. Peptide-based studies exclude antigen processing and presentation biology and may influence T cell detection studies. To focus on responses to whole virus and complex antigens, we used intact SARS-CoV-2 and full-length proteins with DC to activate CD8 and CD4 T cells from convalescent persons. T cell receptor (TCR) sequencing showed partial repertoire preservation after expansion. Resultant CD8 T cells recognize SARS-CoV-2-infected respiratory cells, and CD4 T cells detect inactivated whole viral antigen. Specificity scans with proteome-covering protein/peptide arrays show that CD8 T cells are oligospecific per subject and that CD4 T cell breadth is higher. Some CD4 T cell lines enriched using SARS-CoV-2 cross-recognize whole seasonal coronavirus (sCoV) antigens, with protein, peptide, and HLA restriction validation. Conversely, recognition of some epitopes is eliminated for SARS-CoV-2 variants, including spike (S) epitopes in the alpha, beta, gamma, and delta variant lineages.
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Full text: Available Collection: Preprints Database: medRxiv Type of study: Diagnostic study / Prognostic study / Rct Language: English Year: 2022 Document type: Preprint
Full text: Available Collection: Preprints Database: medRxiv Type of study: Diagnostic study / Prognostic study / Rct Language: English Year: 2022 Document type: Preprint
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