This article is a Preprint
Preprints are preliminary research reports that have not been certified by peer review. They should not be relied on to guide clinical practice or health-related behavior and should not be reported in news media as established information.
Preprints posted online allow authors to receive rapid feedback and the entire scientific community can appraise the work for themselves and respond appropriately. Those comments are posted alongside the preprints for anyone to read them and serve as a post publication assessment.
Longitudinal proteomic investigation of COVID-19 vaccination
Preprint
in English
| medRxiv
| ID: ppmedrxiv-22281744
ABSTRACT
Although the development of COVID-19 vaccines has been a remarkable success, the heterogeneous individual antibody generation and decline over time are unknown and still hard to predict. In this study, blood samples were collected from 163 participants who next received two doses of an inactivated COVID-19 vaccine (CoronaVac(R)) at a 28-day interval. Using TMT-based proteomics, we identified 1715 serum and 7342 peripheral blood mononuclear cells (PBMCs) proteins. We proposed two sets of potential biomarkers (seven from serum, five from PBMCs) using machine learning, and predicted the individual seropositivity 57 days after vaccination (AUC = 0.87). Based on the four PBMCs potential biomarkers, we predicted the antibody persistence until 180 days after vaccination (AUC = 0.79). Our data highlighted characteristic hematological host responses, including altered lymphocyte migration regulation, neutrophil degranulation, and humoral immune response. This study proposed potential blood-derived protein biomarkers for predicting heterogeneous antibody generation and decline after COVID-19 vaccination, shedding light on immunization mechanisms and individual booster shot planning. HighlightsO_LILongitudinal proteomics of PBMC and serum from individuals vaccinated with CoronaVac(R). C_LIO_LIMachine learning models predict neutralizing antibody generation and decline after COVID-19 vaccination. C_LIO_LIThe adaptive and the innate immune responses are stronger in the seropositive groups (especially in the early seropositive group). C_LIO_LIVaccine-induced immunity involves in lymphocyte migration regulation, neutrophil degranulation, and humoral immune response. C_LI
cc_no
Full text:
Available
Collection:
Preprints
Database:
medRxiv
Type of study:
Experimental_studies
/
Prognostic study
/
Rct
Language:
English
Year:
2022
Document type:
Preprint