Low-energy shock wave preconditioning reduces renal ischemic reperfusion injury caused by renal artery occlusion
Acta cir. bras.
; 32(7): 550-558, July 2017. ilus, tab
Article
in En
| VETINDEX
| ID: vti-17587
Responsible library:
BR68.1
Localization: BR68.1
ABSTRACT
Purpose:
To evaluate whether low energy shock wave preconditioning could reduce renal ischemic reperfusion injury caused by renal artery occlusion.Methods:
The right kidneys of 64 male Sprague Dawley rats were removed to establish an isolated kidney model. The rats were then divided into four treatment groups Group 1 was the sham treatment group; Group 2, received only low-energy (12 kv, 1 Hz, 200 times) shock wave preconditioning; Group 3 received the same low-energy shock wave preconditioning as Group 2, and then the left renal artery was occluded for 45 minutes; and Group 4 had the left renal artery occluded for 45 minutes. At 24 hours and one-week time points after reperfusion, serum inducible nitric oxide synthase (iNOS), neutrophil gelatinase-associated lipocalin (NGAL), kidney injury molecule-1 (KIM-1), creatinine (Cr), and cystatin C (Cys C) levels were measured, malondialdehyde (MDA) in kidney tissue was detected, and changes in nephric morphology were evaluated by light and electron microscopy.Results:
Twenty-four hours after reperfusion, serum iNOS, NGAL, Cr, Cys C, and MDA levels in Group 3 were significantly lower than those in Group 4; light and electron microscopy showed that the renal tissue injury in Group 3 was significantly lighter than that in Group 4. One week after reperfusion, serum NGAL, KIM-1, and Cys C levels in Group 3 were significantly lower than those in Group 4.Conclusion:
Low-energy shock wave preconditioning can reduce renal ischemic reperfusion injury caused by renal artery occlusion in an isolated kidney rat model.(AU)Key words
Full text:
1
Database:
VETINDEX
Main subject:
Rats
/
Reperfusion Injury
/
Ischemia
Limits:
Animals
Language:
En
Journal:
Acta cir. bras.
Year:
2017
Document type:
Article