Hepatoprotective effect of Zinc and Magnesium against subchronicCadmium toxicity on biochemical, histopathological andneurobehavioral parameters in Wistar rats

ABSTRACT

The aim of this study was carried out to investigate the possible hepato protective effect of Zinc and Magnesium against subchronic cadmium toxicity on biochemical, histopathological and neurobehavioral parameters in Wistar rats during 90 days. Fourty Wistar rats, were randomly divided in four experimental group 1, control only water; 2, water and oral administration (Cd); 3, (Cd+Mg) in water oral administration; 4, (Cd+Zn) in water oral administration. Zinc and Magnesium was used to reduce hepatoxicity and neurobehavioral disorders induced by Cadmium in Wistar rats. The Cd-Zn and Cd-Mg groups received 10 mg/kg of ZnCl2, 10 mg/kg of MgCl2 added to drinking water. The Cd group received 15mg / kg of CdCl2. Blood glucose level and body weight were measured every day. According to the results, which have been obtained. There was a reduction in body weight gain due to Cd group and an increase liver weight. The results indicated also changes in biochemical parameters, which are characterized by an increase in serum glucose, serum urea, serum creatinine, of animals contaminated with cadmium compared to the control animals. However, the supplementation of (Zn) and Mg combination improved some but not all the previous parameters. Moreover, the findings showed variation in hematological parameters of Cd treated animals. In other word, there was a rise in number of white cells, and reduction of red cells counts, hemoglobin concentration and hematocrit percentage, but all these parameters returned approximately to their normal values after combined Zn and Mg addition. The neurobehavioral test shows an increase in the number of cells crossed, the number of straightening in the test. Open field in the group exposed to Cd resulting in locomotors hyperactivity compared to the control and it is minimal in other groups treated with Zn and Mg...(AU)