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Immunoexpression of proliferating cell nuclear antigen (PCNA) in spleen of splenectomized rats with preservation of inferior pole, submitted to hyperbaric oxygenation
Paulo, Marcela Souza Lima; Santos, Filipy Taifson Barbosa dos; Rocha, Polyanna Gonçalves; Silva, Mirella de Barros; Cintra, Luiz Cálice; Motta, Luciene Lage da; Errera, Flávia Imbroisi Valle; Paulo, Danilo Nagib Salomão; Nunes, Tarcizo Afonso.
Affiliation
  • Paulo, Marcela Souza Lima; Universidade Federal de Minas Gerais. Belo Horizonte. Brasil
  • Santos, Filipy Taifson Barbosa dos; Escola Superior de Ciências da Santa Casa de Misericórdia de Vitória. Faculdade de Ciências da Saúde. Vitória. Brasil
  • Rocha, Polyanna Gonçalves; Escola Superior de Ciências da Santa Casa de Misericórdia de Vitória. Faculdade de Ciências da Saúde. Vitória. Brasil
  • Silva, Mirella de Barros; Escola Superior de Ciências da Santa Casa de Misericórdia de Vitória. Faculdade de Ciências da Saúde. Vitória. Brasil
  • Cintra, Luiz Cálice; Escola Superior de Ciências da Santa Casa de Misericórdia de Vitória. Faculdade de Ciências da Saúde. Vitória. Brasil
  • Motta, Luciene Lage da; Hospital Santa Casa de Misericórdia de Vitória. Vitória. Brasil
  • Errera, Flávia Imbroisi Valle; Escola Superior de Ciências da Santa Casa de Misericórdia de Vitória. Faculdade de Ciências da Saúde. Departamento de Morfologia. Vitória. Brasil
  • Paulo, Danilo Nagib Salomão; Escola Superior de Ciências da Santa Casa de Misericórdia de Vitória. Faculdade de Ciências da Saúde. Departamento de Cirurgia. Vitória. Brasil
  • Nunes, Tarcizo Afonso; Universidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Cirurgia. Belo Horizonte. Brasil
Acta cir. bras. ; 28(10): 691-695, Oct. 2013. ilus, tab
Article in En | VETINDEX | ID: vti-9062
Responsible library: BR1.1
Localization: BR68.1
ABSTRACT

PURPOSE:

To analyze PCNA immunoexpression on the inferior pole of the spleen of splenectomized rats submitted to hyperbaric oxygenation (HBO).

METHODS:

Were analyzed fragments of the inferior pole of the spleen of 20 male Wistar rats submitted to splenectomy with preservation of the inferior pole. The rats were divided in two groups group A (n=10) without HBO and group B (n=10) submitted to HBO at 2, 5 atmospheres per 120 minutes, twice a day for three days and once a day for seven days. The groups were then subdivided in four subgroups A15 (n=5), with euthanasia on the 15th day; A45 (n=5), with euthanasia on the 45th day; B15 (n=5) with euthanasia on the 15th day and B45 with euthanasia on the 45th day. Respectively on these days, fragments of the inferior pole of the spleen of all animals were collected and analyzed with the immunohistochemistry technique in order to evaluate PCNA expression.

RESULTS:

There was an expressive increase in PCNA immunoreactivity in the group B. The 45 day postoperative period resulted in a higher level of positivity than the 15 day postoperative period (p<0.01).

CONCLUSION:

The quantitative analysis of proliferating cell nuclear antigen positive suggests that hyperbaric oxygenation increases cellular proliferation, contributing to splenic regeneration.(AU)
Subject(s)
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Full text: 1 Database: VETINDEX Main subject: Spleen / Hyperbaric Oxygenation Limits: Animals Language: En Journal: Acta cir. bras. Year: 2013 Document type: Article

Full text: 1 Database: VETINDEX Main subject: Spleen / Hyperbaric Oxygenation Limits: Animals Language: En Journal: Acta cir. bras. Year: 2013 Document type: Article