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Efficient production of lignocellulolytic enzymes xylanase, -xylosidase, ferulic acid esterase and -glucosidase by the mutant strain Aspergillus awamori 2B.361 U2/1
Gottschalk, Leda Maria Fortes; Paredes, Raquel de Sousa; Teixeira, Ricardo Sposina Sobral; Silva, Ayla Sant'Ana da; Bon, Elba Pinto da Silva.
Affiliation
  • Gottschalk, Leda Maria Fortes; Embrapa Food Technology. Bioprocess Laboratory. Rio de Janeiro. Brazil
  • Paredes, Raquel de Sousa; Embrapa Food Technology. Bioprocess Laboratory. Rio de Janeiro. Brazil
  • Teixeira, Ricardo Sposina Sobral; Embrapa Food Technology. Bioprocess Laboratory. Rio de Janeiro. Brazil
  • Silva, Ayla Sant'Ana da; Embrapa Food Technology. Bioprocess Laboratory. Rio de Janeiro. Brazil
  • Bon, Elba Pinto da Silva; Embrapa Food Technology. Bioprocess Laboratory. Rio de Janeiro. Brazil
Braz. J. Microbiol. ; 44(2): 569-576, 2013.
Article in En | VETINDEX | ID: vti-967
Responsible library: BR68.1
Localization: BR68.1
ABSTRACT
The production of xylanase, -xylosidase, ferulic acid esterase and -glucosidase by Aspergillus awamori 2B.361 U2/1, a hyper producer of glucoamylase and pectinase, was evaluated using selected conditions regarding nitrogen nutrition. Submerged cultivations were carried out at 30 ºC and 200 rpm in growth media containing 30 g wheat bran/L as main carbon source and either yeast extract, ammonium sulfate, sodium nitrate or urea, as nitrogen sources, in all cases it was used a fixed molar carbon to molar nitrogen concentration of 10.3. The use of poor nitrogen sources favored the accumulation of xylanase, -xylosidase and ferulic acid esterase to a peak concentrations of 44,880, 640 and 118 U/L, respectively, for sodium nitrate and of 34,580, 685 and 170 U/L, respectively, for urea. However, the highest -glucosidase accumulation of 10,470 U/L was observed when the rich organic nitrogen source yeast extract was used. The maxima accumulation of filter paper activity, xylanase, -xylosidase, ferulic acid esterase and -glucosidase by A. awamori 2B.361 U2/1 was compared to that produced by Trichoderma reesei Rut-C30. The level of -glucosidase was over 17-fold higher for the Aspergillus strain, whereas the levels of xylanase and -xylosidase were over 2-fold higher. This strain also produced ferulic acid esterase (170 U/L), which was not detected in the T. reesei culture.(AU)
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Full text: 1 Database: VETINDEX Main subject: Aspergillus / Trichoderma / Endo-1,3(4)-beta-Glucanase Language: En Journal: Braz. J. Microbiol. Year: 2013 Document type: Article
Full text: 1 Database: VETINDEX Main subject: Aspergillus / Trichoderma / Endo-1,3(4)-beta-Glucanase Language: En Journal: Braz. J. Microbiol. Year: 2013 Document type: Article