Comparison of microscopical examination and semi-nested multiplex polymerase chain reaction in diagnosis of Plasmodiumfaldparum and P. vivax
(East. Mediterr. health j).
in English
| WHO IRIS
| ID: who-118078
Responsible library:
CH1.1
ABSTRACT
We compared light microscopy examination and a semi-nested multiplex PCR [SnM-PCR] assay in endemic areas of the Islamic Republic of Iran. A total of 68 individuals with malaria-positive and suspected malaria symptoms were included in the study. Giemsa-stained thick blood films were examined under a light microscope for malaria parasites in 100 and 200 fields. DNA was extracted from blood samples and SnM-PCR based on the amplification of the small subunit ribosomal RNA [ssrRNA] gene sequences was applied. Microscopical examination showed that 48.5% [33.8% P. vivax and 14.7% P.falciparum] and 50% [35.3% P. vivax and 14.7% P.falciparum] of the samples were positive in 100 and 200 fields respectively. SnM-PCR showed the same results as the 200 field microscopy
Full text:
Available
Collection:
Databases of international organizations
Health context:
Neglected Diseases
Health problem:
Malaria
/
Neglected Diseases
Database:
WHO IRIS
Main subject:
Polymerase Chain Reaction
/
Malaria, Vivax
/
Malaria, Falciparum
/
Microscopy
Language:
English
Journal:
East. Mediterr. health j
Year:
2011