Detection of Mycobacterium tuberculosis complex and non-tuberculous mycobacteria by multiplex polymerase chain reactions
(East. Mediterr. health j).
in English
| WHO IRIS
| ID: who-118685
Responsible library:
CH1.1
ABSTRACT
The ability of two-band and three-band multiplex polymerase chain reactions to detect and differentiate Mycobacterium tuberculosis complex from non-tuberculous mycobacteria was evaluated. The polymerase chain reactions differentiated between M. tuberculosis and non-tuberculous mycobacteria when standard strains and clinical isolates of mycobacteria were tested. The sensitivity of the two-band and three-band techniques to detect M. tuberculosis in clinical specimens, compared with smear and/or culture, was 88% and 75% respectively. Although both techniques showed 100% specificity, the superior sensitivity of the two-band technique suggests that it could be more useful in the diagnosis of tuberculosis and in differentiating M. tuberculosis complex from non-tuberculous mycobacteria
Full text:
Available
Collection:
Databases of international organizations
Health context:
Neglected Diseases
Health problem:
Neglected Diseases
/
Tuberculosis
Database:
WHO IRIS
Main subject:
Tuberculosis
/
DNA, Bacterial
/
Polymerase Chain Reaction
/
Reproducibility of Results
/
Sensitivity and Specificity
/
Diagnosis, Differential
/
Mycobacterium
/
Mycobacterium Infections
/
Mycobacterium tuberculosis
Language:
English
Journal:
East. Mediterr. health j
Year:
1999