Cross-Platform Application of DNA Methylation Age Estimation Model in Eastern Chinese Han Population / 法医学杂志
Journal of Forensic Medicine
; (6): 441-446, 2023.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-1009375
Responsible library:
WPRO
ABSTRACT
OBJECTIVES@#To evaluate the forensic application value of an age estimation model based on DNA methylation in eastern Chinese Han population, and to provide a theoretical basis for exploring age estimation models suitable for different detection platforms.@*METHODS@#According to the 6 age-related methylation sites in the published blood DNA methylation age estimation models of Chinese Han population, the DNA methylation level of 48 samples was detected by pyrosequencing and next-generation sequencing (NGS). After submitting DNA methylation levels to the age estimation model, the DNA methylation ages were predicted and compared with their real ages.@*RESULTS@#The 6 DNA methylation sites in both detection techniques were age-related, with an R2 of 0.85 and a median absolute deviation (MAD) of 4.81 years when using pyrosequencing;with an R2 of 0.84 and MAD of 4.41 years when using NGS.@*CONCLUSIONS@#The blood DNA methylation age estimation model can be used under pyrosequencing and multi-purpose regional methylation enrichment sequencing technology based on NGS and it can accurately estimate the age.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Aging
/
CpG Islands
/
DNA Methylation
/
Forensic Genetics
/
East Asian People
Limits:
Humans
Language:
English
Journal:
Journal of Forensic Medicine
Year:
2023
Document type:
Article