Protective effect of Rutin on myelin toxicity in the corpus callosum of rats induced by acrylamide / 解剖学报

ABSTRACT

Objective To study the effect of rutin (Rut) on the expression of myelin basic protein (MBP) and myelin protein lipoprotein (PLP) in corpus callosum of rats infected with acrylamide(ACR). Methods Thirty-two SD adult male rats were randomly divided into 4 groupscontrol,20 mg/ kg acrylamide poisoning group (ACR), 100 mg/ kg Rut protection group (R1+ACR), 200 mg/ kg Rut protection group (R2+ACR),8 in each group,and were given gastric gavage for 21 days. The changes of the rats’ gait were recorded weekly; Immunohistochemistry and Western blotting were used to detect the changes in the expression levels of MBP and PLP in each group of rats. Results The gait score results showed that the gait score of the ACR group increased with the extension of exposure time compared with the control group. The gait score of the R1+ACR group and R2+ACR group also showed an increase trend compared with the control group, but the gait score was significantly lower than that of the ACR group (P<0. 05). Immunohistochemistry and Western blotting results showed that the expression of MBP and PLP in the corpus callosum of the ACR group was significantly decreased compared with the control group (P<0. 01), while the expression of MBP and PLP in the R1+ACR group and R2+ACR group increased (P<0. 05). Conclusion Rutin has a protective effect on myelin sheath in rats infected with acrylamide, which may be related to the inhibition of MBP and PLP in corpus callosum induced by ACR infection.