Differentially expressed genes in the hippocampus of rats with global ischemia : an Affymetrix microarray study / 中华神经医学杂志

ABSTRACT

Objective To screen the differentially expressed genes in the hippocampus of rats with global ischemia. Methods We established the animal models of cardiopulmonary bypass (CPB)in rats, and the rats were divided into 2 groups: rats in control group (n=3) were killed 5 minutes after CPB and rats in ischemia group (n=3) experienced 5-minutes' circulatory arrest. The hippocampal tissue was got from the 2 groups. Affymetrix ratgenome 230 2.0 array was used to detect the gene expression profile of each hippocampus in the 2 groups and the differentially expressed genes were analyzed.Results Fifty-five transcripts (30, up-regulated; 25, down-regulated) in the ischemia group changed; 28transcripts of them changed significantly as compared with those in the control group (P＜0.05).Conclusion Screening the differentially expressed genes may be important in elucidating the molecular biological mechanism of global cerebral ischemic injury.