Rapid Detection and Identification of Human Respiratory Syncytial Virus, Human Parainfluenza Virus Type 1, 2 and 3 by Single-tube Multiplex Reverse Transcription Polymerase Chain Reaction
Journal of Bacteriology and Virology
; : 203-210, 2002.
Article
in Korean
| WPRIM (Western Pacific)
| ID: wpr-105393
Responsible library:
WPRO
ABSTRACT
Laboratory diagnosis of respiratory viral infection has traditionally been based upon virus isolation and/or viral antigen identification. Recently, more sensitive and specific nucleic acid detection methods by reverse transcription- polymerase chain reaction (RT-PCR) have been developed, however, conventional RT-PCR can identify only a single suspected virus. To identify the causative agents which belong to Paramyxoviridae of respiratory virus infections, we have developed a single-tube multiplex RT-PCR using four primer sets which can amplify respiratory syncytial virus and parainfluenza virus type 1, 2 and 3 simultaneously. Assay sensitivity of single-tube multiplex RT-PCR allowed a detection in the range of 3~500 TCID50 and there were no cross amplification among other respiratory viral agents based on the test using reference virus stocks. The single-tube multiplex RT-PCR was able to directly detect viruses in respiratory specimens, with virus being detected 11 of 80 samples as compared to 9 of 80 samples detected by indirect immunofluorescence or antigen detection following shell vial culture. This result suggests that the single-tube multiplex RT-PCR can be established as a more sensitive and rapid diagnostic application than shell vial assay for the detection of respiratory infection of Paramyxoviridae.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Respiratory Syncytial Viruses
/
Paramyxoviridae
/
Polymerase Chain Reaction
/
Respiratory Syncytial Virus, Human
/
Paramyxoviridae Infections
/
Parainfluenza Virus 1, Human
/
Fluorescent Antibody Technique, Indirect
/
Clinical Laboratory Techniques
/
Reverse Transcription
Type of study:
Diagnostic study
Limits:
Humans
Language:
Korean
Journal:
Journal of Bacteriology and Virology
Year:
2002
Document type:
Article