Tumor necrosis factor-alpha deficiency impairs host defense against Streptococcus pneumoniae / 한국실험동물학회지
Laboratory Animal Research
; : 78-85, 2015.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-106542
Responsible library:
WPRO
ABSTRACT
Streptococcus pneumoniae is a major human pathogen that is involved in community-acquired pneumonia. Tumor necrosis factor-alpha (TNF-alpha) is a pro-inflammatory cytokine that activates immune responses against infection, invasion, injury, or inflammation. To study the role of TNF-alpha during S. pneumoniae infection, a murine pneumococcal pneumonia model was used. We intranasally infected C57BL/6J wild-type (WT) and TNF-alpha knockout (KO) mice with S. pneumoniae D39 serotype 2. In TNF-alpha KO mice, continuous and distinct loss of body weight, and low survival rates were observed. Bacterial counts in the lungs and blood of TNF-alpha KO mice were significantly higher than those in WT mice. Histopathological lesions in the spleen of TNF-alpha KO mice were more severe than those in WT mice. In TNF-alpha KO mice, severe depletion of white pulp was observed and the number of apoptotic cells was significantly increased. Interferon-gamma (IFN-gamma), IL-12p70 and IL-10 levels in serum were significantly increased in TNF-alpha KO mice. TNF-alpha is clearly involved in the regulation of S. pneumoniae infections. Early death and low survival rates of TNF-alpha KO mice were likely caused by a combination of impaired bacterial clearance and damage to the spleen. Our findings suggest that TNF-alpha plays a critical role in protecting the host from systemic S. pneumoniae infection.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pneumonia
/
Pneumonia, Pneumococcal
/
Spleen
/
Streptococcus pneumoniae
/
Body Weight
/
Survival Rate
/
Interferon-gamma
/
Tumor Necrosis Factor-alpha
/
Interleukin-10
/
Bacterial Load
Type of study:
Prognostic study
Limits:
Animals
/
Humans
Language:
English
Journal:
Laboratory Animal Research
Year:
2015
Document type:
Article