Comparison of MicroScan and Phoenix Automated Systems for Detection of Extended-Spectrum beta-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae
Laboratory Medicine Online
; : 55-58, 2012.
Article
in Korean
| WPRIM (Western Pacific)
| ID: wpr-106974
Responsible library:
WPRO
ABSTRACT
The aim of this study was to evaluate the performances of MicroScan (Siemens Healthcare, USA) and Phoenix (Becton Dickinson Diagnostic Systems, USA) automated systems for the detection of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae. ESBL-producers were detected from 18 E. coli strains and 26 K. pneumoniae strains using MicroScan, Phoenix, and double-disk synergy test (DDST). The ESBL types were determined by PCR direct sequencing. ESBL genes were detected in 38 (86.4%) of the 44 test strains. The sensitivities of MicroScan, Phoenix, and DDST were 94.6%, 79%, and 89.5%, respectively. Both MicroScan and Phoenix provided acceptable results for the examination of clinical isolates.
Full text:
Available
Health context:
Neglected Diseases
Health problem:
Neglected Diseases
/
Zoonoses
Database:
WPRIM (Western Pacific)
Main subject:
Pneumonia
/
Beta-Lactamases
/
Polymerase Chain Reaction
/
Delivery of Health Care
/
Escherichia
/
Escherichia coli
/
Klebsiella
/
Klebsiella pneumoniae
Type of study:
Diagnostic study
Aspects:
Social determinants of health
Language:
Korean
Journal:
Laboratory Medicine Online
Year:
2012
Document type:
Article