The Relationship between Isoniazid Resistance and 463 CodonMutation of katG Gne in Mycobacterium Tuberculosis / 결핵
Tuberculosis and Respiratory Diseases
; : 8-13, 1996.
Article
in Korean
| WPRIM (Western Pacific)
| ID: wpr-112248
Responsible library:
WPRO
ABSTRACT
BACKGROUND:
The 463 codon mutation of katG gene has been reported as an useful marker for the detection of isoniazid(INH) resistant strains of M. tuberculosis. This study aimed to elucidate relationship between 463 mutation in katG gene and INH resistance in M. tuberculosis.METHOD:
DNA was extracted from 28 INH susceptible strains(MIC > or = 0.2microg/ml on the Lowenstein Jensen media) and used for amplification of 189bp fragment containing 463 codon by PCR. Amplified fragments were digested by restriction enzyme Msp I, analyzed by single strand conformation polymorphism(SSCP) in the MDE gel and sequenced to prove mutation.RESULT:
Only 7(25%) out of 28 were digestible by restriction enzyme Msp I. The SSCP pattern of 21 strains were distinctly different from that of M. tuberculosis H37Rv. Msp I undigestible PCR fragment was substituted at 463 codon from Arg(CGG) to Leu(CTG).CONCLUSION:
This finding clearly indicate no relationship between 463 codon mutation of the katG gene and INH resistance.
Full text:
Available
Health context:
Neglected Diseases
Health problem:
Neglected Diseases
/
Tuberculosis
Database:
WPRIM (Western Pacific)
Main subject:
Tuberculosis
/
Codon
/
DNA
/
Polymerase Chain Reaction
/
Polymorphism, Single-Stranded Conformational
/
Isoniazid
/
Mycobacterium
/
Mycobacterium tuberculosis
Language:
Korean
Journal:
Tuberculosis and Respiratory Diseases
Year:
1996
Document type:
Article